First report of entomopathogenic nematode Steinernema surkhetense and its pathogenic potential to larvae of the Greater Wax Moth (Galleria mellonella L.) in Vietnam

Egyptian Journal of Biological Pest Control

Phap Quang Trinh¹  Duyen Thi Nguyen¹  Linh Thi Mai Le¹  Tien Huu Nguyen² 

[1] Institute of Ecology and Biological Resources, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000, Hanoi, Vietnam;Graduate University of Science and Technology, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000, Hanoi, Vietnam;Institute of Ecology and Biological Resources, Vietnam Academy of Sciences and Technology, 18 Hoang Quoc Viet, Cau Giay, 100000, Hanoi, Vietnam;Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium;

关键词: Entomopathogenic nematodes;    Steinernema surkhetense;    Lethal efficacy;    Galleria mellonella;    Central Highlands, Vietnam;    Biocontrol;   

DOI  :  10.1186/s41938-021-00496-1

来源: Springer

【 摘 要 】

BackgroundEntomopathogenic nematodes (EPNs) are evidently a useful nematode group for the biocontrol of insect pests. It is well known that efficacy of different EPN strains, even belonging to the same species, can be significantly varied in different localities. Therefore, exploring EPNs and testing their efficacy in various ecological regions is of crucial importance to find out more efficient EPN strains. On the other hand, this practice is also needed to enhance the knowledge on diversity and distribution model of EPNs over the world.ResultsIn this study, a species belonging to the genus Steinernema, S. surkhetense, has been characterized for the first time in Vietnam based on morphological and molecular characterizations. Morphological characterizations of infective juveniles, the first and second-generation adults, and molecular characterization of D2-D3 expansion segment of 28S rRNA region were given. Molecular phylogeny of the genus Steinernema was also provided. In addition, the study showed that the lethal efficacy of this local strain to larvae of Galleria mellonella L. was relatively higher than other reported EPN strains in Vietnam.ConclusionsThe Vietnamese EPN population found in this study was determined to be conspecific with S. surkhetense, revealed its new distribution in Vietnam. Besides, detailed morphological and molecular characterizations of it was provided with small variations compared to other populations in the world, and its relatively high lethal efficacy on larvae of G. mellonella implied that this strain can be potentially a useful strain for biological control of insect pests.

【 授权许可】

CC BY