Expression analysis of miRNAs and their putative target genes confirm a preponderant role of transcription factors in the early response of oil palm plants to salinity stress

BMC Plant Biology

André Pereira Leão¹  Carlos Antônio Ferreira de Sousa²  Marcos Mota do Carmo Costa³  Roberto Coiti Togawa³  Priscila Grynberg³  Vivianny Nayse Belo Silva⁴  Fernanda Ferreira Salgado⁴  Letícia Rios Vieira⁴  Manoel Teixeira Souza Júnior⁵ 

[1] Embrapa Agroenergia, CEP 70770-901, Brasília, DF, Brazil;Embrapa Meio Norte, Av. Duque de Caxias, 5650, CEP 64.006-245, Teresina, PI, Brazil;Embrapa Recursos Genéticos e Biotecnologia, CEP 70770-917, Brasília, DF, Brazil;PGBV - Universidade Federal de Lavras – UFLA, CEP 37200-000, Lavras, MG, Brazil;PGBV - Universidade Federal de Lavras – UFLA, CEP 37200-000, Lavras, MG, Brazil;Embrapa Agroenergia, CEP 70770-901, Brasília, DF, Brazil;

关键词: Abiotic stress;    Elaeis guineensis;    Transcription factor;    Transcriptome;    Non-coding RNA;   

DOI  :  10.1186/s12870-021-03296-9

来源: Springer

【 摘 要 】

BackgroundSeveral mechanisms regulating gene expression contribute to restore and reestablish cellular homeostasis so that plants can adapt and survive in adverse situations. MicroRNAs (miRNAs) play roles important in the transcriptional and post-transcriptional regulation of gene expression, emerging as a regulatory molecule key in the responses to plant stress, such as cold, heat, drought, and salt. This work is a comprehensive and large-scale miRNA analysis performed to characterize the miRNA population present in oil palm (Elaeis guineensis Jacq.) exposed to a high level of salt stress, to identify miRNA-putative target genes in the oil palm genome, and to perform an in silico comparison of the expression profile of the miRNAs and their putative target genes.ResultsA group of 79 miRNAs was found in oil palm, been 52 known miRNAs and 27 new ones. The known miRNAs found belonged to 28 families. Those miRNAs led to 229 distinct miRNA-putative target genes identified in the genome of oil palm. miRNAs and putative target genes differentially expressed under salinity stress were then selected for functional annotation analysis. The regulation of transcription, DNA-templated, and the oxidation-reduction process were the biological processes with the highest number of hits to the putative target genes, while protein binding and DNA binding were the molecular functions with the highest number of hits. Finally, the nucleus was the cellular component with the highest number of hits. The functional annotation of the putative target genes differentially expressed under salinity stress showed several ones coding for transcription factors which have already proven able to result in tolerance to salinity stress by overexpression or knockout in other plant species.ConclusionsOur findings provide new insights into the early response of young oil palm plants to salinity stress and confirm an expected preponderant role of transcription factors - such as NF-YA3, HOX32, and GRF1 - in this response. Besides, it points out potential salt-responsive miRNAs and miRNA-putative target genes that one can utilize to develop oil palm plants tolerant to salinity stress.

【 授权许可】

CC BY