Evaluation of the interaction between insecticide resistance-associated genes and malaria transmission in Anopheles gambiae sensu lato in central Côte d’Ivoire

Parasites & Vectors

Matthew B. Thomas¹  Eleanore D. Sternberg²  Simon-Pierre A. Nguetta³  Oulo N’Nan-Alla³  Kpahe M. H. Kanh³  Rosine Z. Wolie⁴  Florent H. A. Yapo⁵  Amal Dahounto⁵  Welbeck A. Oumbouke⁶  Soromane Camara⁷  Alphonsine A. Koffi⁷  Ludovic P. Ahoua Alou⁷  Raphael NGuessan⁸  Innocent Z. Tia⁹ 

[1] Department of Entomology, Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA, USA;Department of Entomology, Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA, USA;Department of Vector Biology, Liverpool School of Tropical Medicine, L3 5QA, Liverpool, UK;Unité de Recherche et de Pédagogie de Génétique, Université Félix Houphouët-Boigny, UFR Biosciences, Abidjan, Côte d’Ivoire;Unité de Recherche et de Pédagogie de Génétique, Université Félix Houphouët-Boigny, UFR Biosciences, Abidjan, Côte d’Ivoire;Vector Control Product Evaluation Centre, Institut Pierre Richet (VCPEC-IPR), Bouaké, Côte d’Ivoire;Institut Pierre Richet (IPR), Institut National de Santé Publique (INSP), Bouaké, Côte d’Ivoire;Vector Control Product Evaluation Centre, Institut Pierre Richet (VCPEC-IPR), Bouaké, Côte d’Ivoire;Vector Control Product Evaluation Centre, Institut Pierre Richet (VCPEC-IPR), Bouaké, Côte d’Ivoire;Innovative Vector Control Consortium, IVCC, Liverpool, UK;Vector Control Product Evaluation Centre, Institut Pierre Richet (VCPEC-IPR), Bouaké, Côte d’Ivoire;Institut Pierre Richet (IPR), Institut National de Santé Publique (INSP), Bouaké, Côte d’Ivoire;Vector Control Product Evaluation Centre, Institut Pierre Richet (VCPEC-IPR), Bouaké, Côte d’Ivoire;Institut Pierre Richet (IPR), Institut National de Santé Publique (INSP), Bouaké, Côte d’Ivoire;Department of Disease Control, London School of Hygiene and Tropical Medicine, London, UK;Vector Control Product Evaluation Centre, Institut Pierre Richet (VCPEC-IPR), Bouaké, Côte d’Ivoire;Institut Pierre Richet (IPR), Institut National de Santé Publique (INSP), Bouaké, Côte d’Ivoire;Université Alassane Ouattara, Bouaké, Côte d’Ivoire;

关键词: Resistance;    Knockdown resistance gene L1014F mutation;    Acetylcholinesterase-1 gene G119S mutation;    Malaria transmission;    Anopheles gambiae;    Anopheles coluzzii;    Côte d’Ivoire;   

DOI  :  10.1186/s13071-021-05079-5

来源: Springer

【 摘 要 】

BackgroundThere is evidence that the knockdown resistance gene (Kdr) L1014F and acetylcholinesterase-1 gene (Ace-1R) G119S mutations involved in pyrethroid and carbamate resistance in Anopheles gambiae influence malaria transmission in sub-Saharan Africa. This is likely due to changes in the behaviour, life history and vector competence and capacity of An. gambiae. In the present study, performed as part of a two-arm cluster randomized controlled trial evaluating the impact of household screening plus a novel insecticide delivery system (In2Care Eave Tubes), we investigated the distribution of insecticide target site mutations and their association with infection status in wild An. gambiae sensu lato (s.l.) populations.MethodsMosquitoes were captured in 40 villages around Bouaké by human landing catch from May 2017 to April 2019. Randomly selected samples of An. gambiae s.l. that were infected or not infected with Plasmodium sp. were identified to species and then genotyped for Kdr L1014F and Ace-1R G119S mutations using quantitative polymerase chain reaction assays. The frequencies of the two alleles were compared between Anopheles coluzzii and Anopheles gambiae and then between infected and uninfected groups for each species.ResultsThe presence of An. gambiae (49%) and An. coluzzii (51%) was confirmed in Bouaké. Individuals of both species infected with Plasmodium parasites were found. Over the study period, the average frequency of the Kdr L1014F and Ace-1R G119S mutations did not vary significantly between study arms. However, the frequencies of the Kdr L1014F and Ace-1R G119S resistance alleles were significantly higher in An. gambiae than in An. coluzzii [odds ratio (95% confidence interval): 59.64 (30.81–131.63) for Kdr, and 2.79 (2.17–3.60) for Ace-1R]. For both species, there were no significant differences in Kdr L1014F or Ace-1R G119S genotypic and allelic frequency distributions between infected and uninfected specimens (P > 0.05).ConclusionsEither alone or in combination, Kdr L1014F and Ace-1R G119S showed no significant association with Plasmodium infection in wild An. gambiae and An. coluzzii, demonstrating the similar competence of these species for Plasmodium transmission in Bouaké. Additional factors including behavioural and environmental ones that influence vector competence in natural populations, and those other than allele measurements (metabolic resistance factors) that contribute to resistance, should be considered when establishing the existence of a link between insecticide resistance and vector competence.Graphical Abstract

【 授权许可】

CC BY