Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

Bioengineered

Bor-Rung Ou¹  Gen-Hung Chen²  Nileema R. Divate³  Rupesh D. Divate³  Yun-Chin Chung³ 

[1] Department of Animal Science and Biotechnology, Tunghai University, Taichung, Republic of China (Taiwan;Department of Cosmetic Science, Providence University, Taichung, Republic of China (Taiwan;Department of Food and Nutrition, Providence University, Taichung, Republic of China (Taiwan;

关键词: Aldehyde reductase;    ethanol production;    neutral trehalase;    Saccharomyces cerevisiae;    Trehalose;    Trehalose-6-phosphate synthase;   

DOI  :  10.1080/21655979.2016.1257449

来源: Taylor & Francis

【 摘 要 】

Lignocellulosic biomass is an attractive low-cost feedstock for bioethanol production. During bioethanol production, Saccharomyces cerevisiae, the common used starter, faces several environmental stresses such as aldehydes, glucose, ethanol, high temperature, acid, alkaline and osmotic pressure. The aim of this study was to construct a genetic recombinant S. cerevisiae starter with high tolerance against various environmental stresses. Trehalose-6-phosphate synthase gene (tps1) and aldehyde reductase gene (ari1) were co-overexpressed in nth1 (coded for neutral trehalase gene, trehalose degrading enzyme) deleted S. cerevisiae. The engineered strain exhibited ethanol tolerance up to 14% of ethanol, while the growth of wild strain was inhibited by 6% of ethanol. Compared with the wild strain, the engineered strain showed greater ethanol yield under high stress condition induced by combining 30% glucose, 30 mM furfural and 30 mM 5-hydroxymethylfurfural (HMF).

【 授权许可】

Unknown