Nuclear envelope mechanobiology: linking the nuclear structure and function

Nucleus

Julianna Goelzer¹  Matthew L. Ferguson²  Matthew Goelzer³  Gunes Uzer⁴  Corey P. Neu⁵ 

[1] Biomolecular Science, Boise State University, Boise, ID, U;Biomolecular Science, Boise State University, Boise, ID, U;Physics, Boise State University, Boise, ID, U;Materials Science and Engineering, Boise State University, Boise, ID, U;Mechanical and Biomedical Engineering, Boise State University, Boise, ID, U;Paul M. Rady Department of Mechanical Engineering, University of Colorado, Boulder, CO, U;

关键词: Nuclear envelope;    nuclear mechanics;    mechanobiology;    chromatin;    live imaging;   

DOI  :  10.1080/19491034.2021.1962610

来源: Taylor & Francis

【 摘 要 】

The nucleus, central to cellular activity, relies on both direct mechanical input as well as its molecular transducers to sense external stimuli and respond by regulating intra-nuclear chromatin organization that determines cell function and fate. In mesenchymal stem cells of musculoskeletal tissues, changes in nuclear structures are emerging as a key modulator of their differentiation and proliferation programs. In this review we will first introduce the structural elements of the nucleoskeleton and discuss the current literature on how nuclear structure and signaling are altered in relation to environmental and tissue level mechanical cues. We will focus on state-of-the-art techniques to apply mechanical force and methods to measure nuclear mechanics in conjunction with DNA, RNA, and protein visualization in living cells. Ultimately, combining real-time nuclear deformations and chromatin dynamics can be a powerful tool to study mechanisms of how forces affect the dynamics of genome function.

【 授权许可】

CC BY