Sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection

Virulence

Juan Zhang¹  Fan Jia¹  Daiying Li²  Junying Chen²  Kai Feng²  Mingwang Long²  Lingmei Yan²  Yue Pan²  Han Wang²  Qiangming Sun²  Xiaodan Wang²  Xuelei Ning³  Lijuan Qiu⁴ 

[1] Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, Peoples Republic of Chin;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming, PR Chin;Kunming Medical University, Kunming, Peoples Republic of Chin;Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, Peoples Republic of Chin;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming, PR Chin;Yunnan Key Laboratory of Vector-borne Infectious Disease, Kunming, Peoples Republic of Chin;Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, Peoples Republic of Chin;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming, PR Chin;Yunnan University, Kunming, Peoples Republic of Chin;Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming, PR Chin;

关键词: DENV-3 (dengue virus serotype 3);    ade (antibody-dependent enhancement);    high-throughput sequencing;    rna-seq;    differentially expressed rnas (de rnas);    rnas expression profile;    denv-host interactions;    immune system;    viral infectious diseases;    interferon-stimulated genes;   

DOI  :  10.1080/21505594.2021.1996072

来源: Taylor & Francis

【 摘 要 】

Dengue virus infection mainly causes dengue hemorrhagic fever (DHF) and/or dengue shock syndrome (DSS). However, ADE (antibody-dependent enhancement) is one of the main pathogenic factors, and its pathogenic mechanism has not been fully elucidated. Recently, with the development of high-throughput sequencing, an increased number of RNAs have been confirmed to play a vital regulatory role in the process of virus infection. However, there is a lack of research on dengue virus infection and ADE. In this study, we used RNA-Seq to detect differentially expressed RNAs (DE RNAs) profiles in mock-infected, DENV-3-infected, and ADE-infected THP-1 cells. Firstly, we found 69 circRNAs, 259 miRNAs, and 18 mRNAs were differentially expressed in THP-1 vs DENV-3. In THP-1 vs ADE, 94 circRNAs, 263 miRNAs, and 111 mRNAs were differentially expressed. In DENV-3 vs ADE, 68 circRNAs, 105 miRNAs, and 94 mRNAs were differentially expressed. Functional enrichment analysis of these DE RNAs mainly focused on immune system, viral infectious diseases, cytokine-cytokine receptor interactions, and NOD/RIG-I-like receptor signaling pathways. In DENV-3 vs ADE, notably, the expression of HBB was up-regulated, which was a Fcγ Receptor-mediated phagocytosis protein. Additionally, we predicted the encoding ability of DE circRNAs, and it was found that a small peptide was encoded by novel_circ_001562 and that its amino acid sequence was consistent with that of DDX60L, which is a class of interferon-stimulated genes. Finally, we constructed the ceRNA regulatory network pathway. Therefore, our study provides a new strategy for further investigation on DENV-host interactions.

【 授权许可】

CC BY