Interferon alpha-inducible protein 27 (IFI27) is a prognostic marker for pancreatic cancer based on comprehensive bioinformatics analysis

Bioengineered

Yalian Sa¹  Rongxia Zuo¹  Yanqiong Li²  Zhihui Ma³  Shu Huang⁴  Jianxin Song⁴  Hongmei OuYang⁴  Jinglin Zhao⁴ 

[1] Center for Clinical Medicine Research (Yunnan Provincial Key Laboratory of Clinical Virology), The First People’s Hospital of Yunnan Province, Kunming, Yunnan, Chin;Central Sterile Supply Department, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, Chin;Institute of Ageing Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang, Chin;The Affiliated Hospital of Kunming University of Science and Technology, Kunming, Yunnan, Chin;Institute of Laboratory Medicine, The First People’s Hospital of Yunnan Province, Kunming, Yunnan, Chin;

关键词: Interferon alpha-inducible protein 27 (IFI27);    pancreatic cancer;    tumor immune microenvironment (TIME);    glycolysis;    prognosis;   

DOI  :  10.1080/21655979.2021.1985858

来源: Taylor & Francis

【 摘 要 】

Accurate biomarkers to predict the genesis and progression of pancreatic adenocarcinoma (PAAD) are needed in the fight against this deadly disease. Here, we combined multiple datasets (GEO, TCGA and GTEx) to conduct a comprehensive analysis of pancreatic cancer. Through an in-depth analysis, we discovered that the expression of the gene encoding interferon alpha-inducible protein 27 (IFI27) was significantly higher in pancreatic cancer tissues than that in normal tissues, and that higher expression of IFI27 was negatively correlated with the overall survival rate of pancreatic cancer patients. The functional annotation of IFI27 demonstrated relationships to cellular immunity and metabolism, especially glycolysis. Analysis of infiltrating immune cells displayed that higher expression of IFI27 expression correlates with decreased CD8 + T cells and increased M2 macrophages in the tumor immune microenvironment (TIME), then biochemical analyses of a mouse model and immunohistochemical (IHC) staining verified that glycolytic enzymes and M2 macrophages increased significantly in pancreatic cancer tissues. We speculate that IFI27 may affect the tumor microenvironment (TME) of PAAD by regulating cellular immunity and metabolism, thereby promoting the progression of pancreatic carcinoma and worsening the prognosis. These findings of our present study are solid evidence that IFI27 is a potential prognostic biomarker of pancreatic cancer and that it affects the tumor immune microenvironment.

【 授权许可】

CC BY