Identification of microRNA hsa-miR-30c-5p as an inhibitory factor in the progression of hepatocellular carcinoma and investigation of its regulatory network via comprehensive analysis

Bioengineered

Chuanmiao Liu¹  Xiaoyu Fang¹  Dongdong Li¹  Li Zhang¹  Jiasheng Chen¹  Shangshang Hu²  Guoqing Guo³  Jing Dai³  Zhiyong Sheng³  Jinyan Zhang⁴  Yu Gao⁴ 

[1] Department of Infectious Diseases, First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Bengbu, Chin;National Clinical Research Center for Infectious Diseases, First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Bengbu, Chin;School of Laboratory Medicine, Research Center of Clinical Laboratory Science, School of Laboratory Medicine, Bengbu Medical College, Bengbu, Chin;School of Life Science, Bengbu Medical College, Bengbu, Chin;School of Life Science, Bengbu Medical College, Bengbu, Chin;Anhui Province Key Laboratory of Translational Cancer Research, Bengbu Medical College, Bengbu, Chin;

关键词: Hsa-miR-30c-5p;    hepatocellular carcinoma;    miRNA;    lncRNAs;    ceRNA regulatory network;    inhibitory factor;   

DOI  :  10.1080/21655979.2021.1979439

来源: Taylor & Francis

【 摘 要 】

Hepatocellular carcinoma (HCC) is a primary liver cancer with high morbidity and mortality. An increasing number of abnormal gene expressions were identified to be associated with the progression of HCC. Previous studies showed that the hsa-miR-30 c-5p (miR-30 c), one of the miR-30 family members, might play a role in suppressing tumor progression in a variety of tumors. The present study aims to examine miR-30 c effects in the development of HCC. The role of miR-30 c in HCC was comprehensively investigated by using bioinformatics and experiments in vitro. The multiple databases were combined to predict and screen the target genes and upstream lncRNAs of miR-30 c, and then constructed a competitive endogenous RNA (ceRNA) regulatory network with miR-30 c as the central miRNA. The miR-30 c-related ceRNA regulatory network was also initially validated in vitro. The results showed that miR-30 c over-expression could inhibit proliferation, migration, invasion, induce apoptosis, and increase G0/G1 phase ratio of HCC cells. Three miR-30 c upstream lncRNAs and 12 miR-30 c target genes were expressed in HCC cells with increased expression and poor prognosis, and a miR-30 C-related ceRNA regulatory network was constructed. This study verified miR-30 c as an inhibitory factor in the progression of HCC and performed analyses on the miR-30 c regulatory network, which might provide potential target information for HCC prognoses and therapies. However, further experiments in vivo and studies including clinical trials will be conducted to validate our results.

【 授权许可】

CC BY