Glucoside xylosyltransferase 2 as a diagnostic and prognostic marker in gastric cancer via comprehensive analysis

Bioengineered

Yunxia Zhao¹  Mulin Liu²  Shuanhu Wang²  Zhaogen Cai³  Shangshang Hu⁴  Jing Dai⁵  Jinyan Zhang⁶  Yu Gao⁶ 

[1] Department of Basic Medical College, Bengbu Medical College, Bengbu, Chin;Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui, Chin;Department of Pathology, First Affiliated Hospital of Bengbu Medical College, Bengbu Medical College, Bengbu, Chin;Research Center of Clinical Laboratory Science, School of Laboratory Medicine, Bengbu Medical College, Bengbu, Chin;School of Life Science, Bengbu Medical College, Bengbu, Chin;School of Life Science, Bengbu Medical College, Bengbu, Chin;Anhui Province Key Laboratory of Translational Cancer Research, Bengbu Medical College, Bengbu, Chin;

关键词: Glucoside xylosyltransferase 2 (GXYLT2);    gastric cancer (GC);    prognosis;    overall survival;    immune cell infiltration;    bioinformatics analysis;   

DOI  :  10.1080/21655979.2021.1967067

来源: Taylor & Francis

【 摘 要 】

To investigate the potential role of GXYLT2 (glucoside xylosyltransferase 2) in gastric cancer (GC), the TCGA (The Cancer Genome Atlas) database and Gene Expression Omnibus (GEO) dataset were used to evaluate GXYLT2 mRNA expression, and the standardized mean difference and diagnostic value were comprehensively assessed. Survival analysis and univariate/multivariate cox regression analysis were performed to evaluate the prognostic value of GXYLT2 in GC patients. The correlation between GXYLT2 and tumor immune cells was identified by using the CIBERSORT algorithm. The results showed that GXYLT2 expression level was significantly increased in GC tissues. GXYLT2 expression was significantly correlated with the grade, stage, and invasion depth of gastric cancer. Overall survival was reduced in the high GXYLT2 expression group. Univariate and multivariate Cox regression analyses showed that GXYLT2 was a reliable prognostic factor. GSEA showed that GXYLT2 might participate in the development of GC through tumor-related pathways. The expression of GXYLT2 was positively correlated with 5 tumor-infiltrating immune cells (resting dendritic cells, m2 macrophages, monocytes, active NK cells and resting mast cells), and was negatively correlated with 6 tumor-infiltrating immune cells (plasma cells, activated memory CD4 T cells, resting NK cells, activated dendritic cells, and activated neutrophils and mast cells). Through cell experiment verification, GXYLT2 expression level in gastric cancer cells was found to be high, which verified the results from the bioinformatics analysis. Furthermore, immunohistochemical staining results also showed that GC tissues had positive GXYLT2 expression. In summary, GXYLT2 might be a potential diagnostic and prognostic biomarker for gastric cancer.

【 授权许可】

CC BY