Circular RNA circ_0008274 upregulates granulin to promote the progression of hepatocellular carcinoma via sponging microRNA -140-3p

Bioengineered

Yazhou Wen¹  Xinhua Zhu²  Chanchan Gao³  Xuyu Gu³  Feng Jiang³ 

[1] Department of Anesthesiology, Zhongda Hospital Affiliated to Southeast University, Nanjing, Chin;Department of General Surgery, Nanjing Drum Tower Hospital, Nanjing, Chin;Department of Oncology, Zhongda Hospital Affiliated to Southeast University, Nanjing, Chin;

关键词: HCC;    circRNA;    microRNA;    granulin;   

DOI  :  10.1080/21655979.2021.1926195

来源: Taylor & Francis

【 摘 要 】

Circular RNA (circRNA) features prominently in the progression of hepatocellular carcinoma (HCC), of which the biological function and potential mechanism of circ_0008274 in HCC are obscure. The present study aims to explore circ_ 0008274’s biological functions and underlying mechanisms in HCC. The expressions of circ_0008274, miR-140-3p and Granulin (GRN) mRNA in HCC tissues and cells were investigated by quantitative real-time polymerase chain reaction. Besides, GRN protein expression was measured by Western blot. Furthermore, chi-square test was used to probe the interrelation between circ_0008274 expression and clinicopathological parameters. In addition, cell counting kit-8 (CCK-8) and EdU assays were applied to detect cell proliferation. Moreover, transwell assay was used to detect cell migration and invasion. What’s more, bioinformatics prediction, dual-luciferase reporter gene assay and RNA Immunoprecipitation experiments were used to corroborate the targeting interrelations among circ_0008274, miR-140-3p and GRN. Herein we reported that circ_0008274 was highly expressed in HCC, and its high expression enhanced the proliferation, migration, and invasion of HCC cells, while depleting circ_0008274 inhibited the malignant biological behaviors of HCC cells. Mechanistically, circ_0008274 upregulates GRN expressions via adsorbing miR-140-3p to expedite the progression of HCC.

【 授权许可】

CC BY