A clubroot pathogen effector targets cruciferous cysteine proteases to suppress plant immunity

Virulence

Md Musharaf Hossain¹  Christopher D. Todd¹  Yangdou Wei¹  Peta C Bonham-Smith¹  Edel Pérez-López² 

[1] Department of Biology, University of Saskatchewan, Saskatoon, Canad;Department of Biology, University of Saskatchewan, Saskatoon, Canad;Department of Plant Sciences, University Laval, Criv, Quebec City, Canad;

关键词: Cysteine protease inhibitor;    papain-like cysteine proteases (plcps);    apoplast;    clubroot;    plant defense;    plasmodiophora brassicae;   

DOI  :  10.1080/21505594.2021.1968684

来源: Taylor & Francis

【 摘 要 】

Plant pathogen effector proteins are key to pathogen virulence. In susceptible host Brassicas, the clubroot pathogen, Plasmodiophora brassicae, induces the production of nutrient-sink root galls, at the site of infection. Among a list of 32 P. brassiae effector candidates previously reported by our group, we identified SSPbP53 as a putative apoplastic cystatin-like protein highly expressed during the secondary infection. Here we found that SSPbP53 encoding gene is conserved among several P. brassicae pathotypes and that SSPbP53 is an apoplastic protein able to directly interact with and inhibit cruciferous papain-like cysteine proteases (PLCPs), specifically Arabidopsis XYLEM CYSTEINE PEPTIDASE 1 (AtXCP1). The severity of clubroot disease is greatly reduced in the Arabidopsis xcp1 null mutant (AtΔxcp1) after infection with P. brassicae resting spores, indicating that the interaction of P. brassicae SSPbP53 with XCP1 is important to clubroot susceptibility. SSPbP53 is the first cystatin-like effector identified and characterized for a plant pathogenic protist.

【 授权许可】

CC BY