CRISPR–Cas9-based genetic engineering for crop improvement under drought stress

Bioengineered

Saheera Tazein¹  Ayesha Arshad²  Ya Ping Chen³  Xiao Tian Zhu³  Abdul Sami³  Ke Jin Zhou³  Zong He Zhu³  Zhao Xue³  Yue Hong³ 

[1] Pgrl CABB, University of Agriculture Faisalabad, Faisalabad, Pakista;Plant Physiology Lab, Quaid I Azam University, Islamabad, Pakista;Rapeseed Cultivation and Breeding Lab, Anhui Agricultural University, Hefei, Chin;

关键词: Drought;    CRISPR–Cas9;    plant productivity;    ABA regulation;    ethylene;    genome editing;   

DOI  :  10.1080/21655979.2021.1969831

来源: Taylor & Francis

【 摘 要 】

In several parts of the world, the prevalence and severity of drought are predicted to increase, creating considerable pressure on global agricultural yield. Among all abiotic stresses, drought is anticipated to produce the most substantial impact on soil biota and plants, along with complex environmental impacts on other ecological systems. Being sessile, plants tend to be the least resilient to drought-induced osmotic stress, which reduces nutrient accessibility due to soil heterogeneity and limits nutrient access to the root system. Drought tolerance is a complex quantitative trait regulated by multiple genes, and it is one of the most challenging characteristics to study and classify. Fortunately, the clustered regularly interspaced short palindromic repeat (CRISPR) technology has paved the way as a new frontier in crop improvement, thereby revolutionizing plant breeding. The application of CRISPER systems has proven groundbreaking across numerous biological fields, particularly in biomedicine and agriculture. The present review highlights the principle and optimization of CRISPR systems and their implementation for crop improvement, particularly in terms of drought tolerance, yield, and domestication. Furthermore, we address the ways in which innovative genome editing tools can help recognize and modify novel genes coffering drought tolerance. We anticipate the establishment of effective strategies of crop yield improvement in water-limited regions through collaborative efforts in the near future.

【 授权许可】

CC BY