Enamel matrix derivative (EMD) enhances the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs)

Bioengineered

ZhaoYang Ye¹  HongChao Feng²  Lu Cheng³  Yi Luo³  Xin Chen⁴  MaoHua Meng⁴  Qiang Dong⁵  ZhengLong Tang⁵  Ying Li⁶  HeLin Chen⁶  Xiao Zeng⁶  Qian Xia⁶ 

[1] Clinical Research Center, the Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, 550004, People’s Republic of Chin;Department of Oral and Maxillofacial Surgery, Guiyang Hospital of Stomatology, Guiyang, Guizhou Province, 550002, People’s Republic of Chin;Department of Prosthodontics, Guiyang Hospital of Stomatology, Guiyang, Gsuizhou Province, 550002, People’s Republic of Chin;Department of Prosthodontics, School of Stomatology, Guizhou Medical University, Guiyang, Guizhou Province, 550004, People’s Republic of Chin;Department of Prosthodontics, School of Stomatology, Guizhou Medical University, Guiyang, Guizhou Province, 550004, People’s Republic of Chin;Department of Prosthodonticsand Oral Implantology, Stomatological hospital of Guizhou Medical University, Guiyang, Guizhou Province, 550004, People’s Republic of Chin;Department of Prosthodonticsand Oral Implantology, Stomatological hospital of Guizhou Medical University, Guiyang, Guizhou Province, 550004, People’s Republic of Chin;

关键词: Enamel matrix derivative;    bone marrow mesenchymal stem cells;    Wnt/β-catenin signaling;   

DOI  :  10.1080/21655979.2021.1971504

来源: Taylor & Francis

【 摘 要 】

To investigate the EMD’s capacity in BMSCs osteogenic differentiation. In vivo and in vitro, BMSCs were treated with EMD, scanning electron microscopy, and Alizarin Red staining were used to detect the changes in the osteogenic ability of BMSCs, and the proliferation ability of BMSCs was evaluated by CCK8. In addition, by adding xav939, a typical inhibitor of Wnt/β-catenin signaling pathway, the regulatory function of Wnt/β-catenin signaling was clarified. The results showed that EMD promote cell proliferation and 25 μg/ml EMD had the most significant effect. Cells inducing osteogenesis for 2 and 3 even 4 weeks, the cell staining is deeper in EMD treated group than that of the control (P < 0.05) by alizarin Red staining, suggesting more mineralization of BMSCs. In vivo implanting the titanium plate wrapped with 25 μg/ml EMD treated-BMSC film into nude mice for 8 weeks, more nodules were formed on the surface of the titanium plate than that the control (P < 0.05). HE showed that there is a little blue-violet immature bone-like tissue block. Besides, the expression of RUNX Family Transcription Factor 2 (Runx2), Osterix, Osteocalcin (OCN), collagen I (COLI), alkaline phosphatase (ALP) and β-catenin were inhibited in xav939 group (P < 0.05); Inversely, all were activated in EMD group (P < 0.05). In conclusion, EMD promoted the proliferation and osteogenic differentiation of BMSCs. EMD’s function on BMSCs might be associated with the Wnt/β-catenin signaling pathway.

【 授权许可】

CC BY