Aminoglycoside riboswitch control of the expression of integron associated aminoglycoside resistance adenyltransferases

Virulence

Xuhui Zhang¹  Getong Liu¹  Yaowen Chang¹  Shasha Wang¹  Dongrong Chen¹  Alastair I.H. Murchie¹  Weizhi He¹  Jun Zhang¹  Wenxia Sun¹ 

[1] Key Laboratory of Medical Epigenetics and Metabolism, Fudan University Pudong Medical Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, PR Chin;Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences, Fudan University, Shanghai, PR Chin;

关键词: Aminoglycoside;    Riboswitch;    Adenyltransferase;    Antibiotic Resistance;   

DOI  :  10.1080/21505594.2020.1836910

来源: Taylor & Francis

【 摘 要 】

The proliferation of antibiotic resistance has its origins in horizontal gene transfer. The class 1 integrons mediate gene transfer by assimilating antibiotic-resistance genes through site-specific recombination. For the class 1 integrons the first assimilated gene normally encodes an aminoglycoside antibiotic resistance protein which is either an aminoglycoside acetyltransferase (AAC), nucleotidyltransferase – (ANT), or adenyl transferase (AAD). An aminoglycoside-sensing riboswitch RNA in the leader RNA of AAC/AAD that controls the expression of aminoglycoside resistance genes has been previously described. Here we explore the relationship between the recombinant products of integron recombination and a series of candidate riboswitch RNAs in the 5ʹ UTR of aad (aminoglycoside adenyltransferases) genes. The RNA sequences from the 5ʹ UTR of the aad genes from pathogenic strains that are the products of site-specific DNA recombination by class 1 integrons were investigated. Reporter assays, MicroScale Thermophoresis (MST) and covariance analysis revealed that a functional aminoglycoside-sensing riboswitch was selected at the DNA level through integron-mediated site-specific recombination. This study explains the close association between integron recombination and the aminoglycoside-sensing riboswitch RNA.

【 授权许可】

CC BY