Simultaneous knockout of multiple LHCF genes using single sgRNAs and engineering of a high‐fidelity Cas9 for precise genome editing in marine algae

Plant Biotechnology Journal

Per Winge¹  Marianne Nymark¹  Amit K. Sharma¹  Snorre Flo¹  Torfinn Sparstad¹  Atle M. Bones¹ 

[1] Cell, Molecular Biology and Genomics Group, Department of Biology, Norwegian University of Science and Technology, NTNU, Trondheim, Norway;

关键词: Light‐harvesting complex proteins;    High‐Fidelity (HiFi) Cas9 nuclease;    precision genome editing;    off‐target gene editing;    lhcf;    diatom;    Phaeodactylum tricornutum;   

DOI  :  10.1111/pbi.13582

来源: Wiley

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CC BY