Biosynthesis of lactones from diols mediated by an artificial flavin

Bioresources and Bioprocessing

Mengjiao Xu¹  Zhuotao Tan¹  Wei Zhuang¹  Hanjie Ying¹  Chenjie Zhu¹  Dong Liu¹  Ming Li¹  Xiaowang Zhang¹  Siyu Qi²  Wenlong Xiong²  Chaojian Li³ 

[1] College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China;School of Life Sciences, Zhengzhou University, Zhengzhou, China;Technology Center, China Tobacco Jiangsu Industry Co., Ltd., 210019, Nanjing, Jiangsu, China;

关键词: Lactones;    Diols;    Cofactor regeneration;    Flavin;    Biosynthesis;   

DOI  :  10.1186/s40643-021-00450-x

来源: Springer

【 摘 要 】

BackgroundLactones are important compounds in the field of medicine, material and chemical industry. One of the promising accesses to these flexible scaffolds is NAD(P)+-dependent alcohol dehydrogenases-catalyzed oxidative lactonization of diols, which relies on the construction of an efficient NAD(P)+ regeneration system.ResultsIn this study, a novel system combining horse liver alcohol dehydrogenase (HLADH) with the synthetic bridged flavin cofactor was established for biosynthesis of lactones. The reaction conditions of this system were optimized and a variety of lactones including chiral lactones were efficiently obtained from various diols. Compared to the previously reported NAD(P)+-regeneration systems, this system showed better regeneration efficiency and product yield. A two-phase system was further applied to solve the problem of product inhibition, and 80% yield was obtained at the condition of 300 mM substrate.ConclusionsThis study provides an efficient method to synthesis of lactones from diols under mild conditions. We believe this system will be a promising alternative to promote the synthesis of other valuable compounds.Graphic abstract

【 授权许可】

CC BY