Single-cell analysis of the ventricular-subventricular zone reveals signatures of dorsal and ventral adult neurogenesis

eLife

Ricardo Romero Rodriguez¹  Daniel A Lim¹  Arturo Álvarez-Buylla¹  Marcos Assis Nascimento¹  Kirsten Obernier¹  Stephanie A Redmond¹  Arantxa Cebrian-Silla¹  David Wu²  Benjamin Mansky³  Susana Gonzalez-Granero⁴  Jose Manuel García-Verdugo⁴ 

[1] Eli and Edythe Broad Institute for Stem Cell Research and Regeneration Medicine, University of California, San Francisco, San Francisco, United States;Department of Neurological Surgery, University of California, San Francisco, San Francisco, United States;Eli and Edythe Broad Institute for Stem Cell Research and Regeneration Medicine, University of California, San Francisco, San Francisco, United States;Department of Neurological Surgery, University of California, San Francisco, San Francisco, United States;Medical Scientist Training Program, University of California, San Francisco, San Francisco, United States;Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, United States;Eli and Edythe Broad Institute for Stem Cell Research and Regeneration Medicine, University of California, San Francisco, San Francisco, United States;Department of Neurological Surgery, University of California, San Francisco, San Francisco, United States;Neuroscience Graduate Program, University of California, San Francisco, San Francisco, United States;Laboratorio de Neurobiología Comparada, Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València - Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Paterna, Spain;

关键词: adult neurogenesis;    single-cell sequencing;    olfactory bulb;    regional differences;    neuroblast;    stem cell;    Mouse;   

DOI  :  10.7554/eLife.67436

来源: eLife Sciences Publications, Ltd

【 摘 要 】

The ventricular-subventricular zone (V-SVZ), on the walls of the lateral ventricles, harbors the largest neurogenic niche in the adult mouse brain. Previous work has shown that neural stem/progenitor cells (NSPCs) in different locations within the V-SVZ produce different subtypes of new neurons for the olfactory bulb. The molecular signatures that underlie this regional heterogeneity remain largely unknown. Here, we present a single-cell RNA-sequencing dataset of the adult mouse V-SVZ revealing two populations of NSPCs that reside in largely non-overlapping domains in either the dorsal or ventral V-SVZ. These regional differences in gene expression were further validated using a single-nucleus RNA-sequencing reference dataset of regionally microdissected domains of the V-SVZ and by immunocytochemistry and RNAscope localization. We also identify two subpopulations of young neurons that have gene expression profiles consistent with a dorsal or ventral origin. Interestingly, a subset of genes are dynamically expressed, but maintained, in the ventral or dorsal lineages. The study provides novel markers and territories to understand the region-specific regulation of adult neurogenesis.

【 授权许可】

CC BY