Tissue-specific targeting of DNA nanodevices in a multicellular living organism

eLife

Franck Perez¹  Sandhya P Koushika²  Sunaina Surana³  Yamuna Krishnan³  Palapuravan Anees³  Kasturi Chakraborty³  Simona Martin³  Jihad Aburas⁴  Paschalis Kratsios⁵  Sandrine Moutel⁶ 

[1] Cell Biology and Cancer Unit, Institut Curie, PSL Research University, CNRS UMR144, Paris, France;Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, India;Department of Chemistry, The University of Chicago, Chicago, United States;Grossman Institute of Neuroscience, Quantitative Biology and Human Behavior, The University of Chicago, Chicago, United States;Department of Neurobiology, The University of Chicago, Chicago, United States;Grossman Institute of Neuroscience, Quantitative Biology and Human Behavior, The University of Chicago, Chicago, United States;Department of Neurobiology, The University of Chicago, Chicago, United States;Recombinant Antibody Platform (TAb-IP), Institut Curie, PSL Research University, CNRS UMR144, Paris, France;Cell Biology and Cancer Unit, Institut Curie, PSL Research University, CNRS UMR144, Paris, France;

关键词: DNA nanotechnology;    endocytosis;    lysosomes;    cell membrane;    live imaging;    nematodes;    C. elegans;   

DOI  :  10.7554/eLife.67830

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Nucleic acid nanodevices present great potential as agents for logic-based therapeutic intervention as well as in basic biology. Often, however, the disease targets that need corrective action are localized in specific organs, and thus realizing the full potential of DNA nanodevices also requires ways to target them to specific cell types in vivo. Here, we show that by exploiting either endogenous or synthetic receptor-ligand interactions and leveraging the biological barriers presented by the organism, we can target extraneously introduced DNA nanodevices to specific cell types in Caenorhabditis elegans, with subcellular precision. The amenability of DNA nanostructures to tissue-specific targeting in vivo significantly expands their utility in biomedical applications and discovery biology.

【 授权许可】

CC BY