Circadian regulation of vertebrate cone photoreceptor function

eLife

Christian Grimm¹  Marijana Samardzija¹  Jennifer Keim²  Stephan CF Neuhauss²  Jingjing Zang²  Matthias Gesemann² 

[1] Lab for Retinal Cell Biology, Department of Ophthalmology, University Hospital Zurich, University of Zurich, Zurich, Switzerland;University of Zurich, Department of Molecular Life Sciences, Zurich, Switzerland;

关键词: circadian rhythm;    visual transduction cascade;    retina;    electroretinography;    visual behavior;    Mouse;    Zebrafish;   

DOI  :  10.7554/eLife.68903

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Eukaryotes generally display a circadian rhythm as an adaption to the reoccurring day/night cycle. This is particularly true for visual physiology that is directly affected by changing light conditions. Here we investigate the influence of the circadian rhythm on the expression and function of visual transduction cascade regulators in diurnal zebrafish and nocturnal mice. We focused on regulators of shut-off kinetics such as Recoverins, Arrestins, Opsin kinases, and Regulator of G-protein signaling that have direct effects on temporal vision. Transcript as well as protein levels of most analyzed genes show a robust circadian rhythm-dependent regulation, which correlates with changes in photoresponse kinetics. Electroretinography demonstrates that photoresponse recovery in zebrafish is delayed in the evening and accelerated in the morning. Functional rhythmicity persists in continuous darkness, and it is reversed by an inverted light cycle and disrupted by constant light. This is in line with our finding that orthologous gene transcripts from diurnal zebrafish and nocturnal mice are often expressed in an anti-phasic daily rhythm.

【 授权许可】

CC BY