The cell adhesion molecule Sdk1 shapes assembly of a retinal circuit that detects localized edges

eLife

Catherine Theriault¹  Pierre-Luc Rochon¹  Arjun Krishnaswamy¹  Aline Giselle Rangel Olguin¹ 

[1] Department of Physiology, McGill University, Montreal, Canada;

关键词: retinal circuitry;    neurodevelopment;    calcium imaging;    two-photon;    immunoglobulin superfamily;    retinal ganlgion cells;    Mouse;   

DOI  :  10.7554/eLife.70870

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Nearly 50 different mouse retinal ganglion cell (RGC) types sample the visual scene for distinct features. RGC feature selectivity arises from their synapses with a specific subset of amacrine (AC) and bipolar cell (BC) types, but how RGC dendrites arborize and collect input from these specific subsets remains poorly understood. Here we examine the hypothesis that RGCs employ molecular recognition systems to meet this challenge. By combining calcium imaging and type-specific histological stains, we define a family of circuits that express the recognition molecule Sidekick-1 (Sdk1), which include a novel RGC type (S1-RGC) that responds to local edges. Genetic and physiological studies revealed that Sdk1 loss selectively disrupts S1-RGC visual responses, which result from a loss of excitatory and inhibitory inputs and selective dendritic deficits on this neuron. We conclude that Sdk1 shapes dendrite growth and wiring to help S1-RGCs become feature selective.

【 授权许可】

CC BY