Ciliary and extraciliary Gpr161 pools repress hedgehog signaling in a tissue-specific manner

eLife

Bandarigoda N Somatilaka¹  Sun-Hee Hwang¹  Kevin White¹  Saikat Mukhopadhyay¹ 

[1] Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, United States;

关键词: cilia;    hedgehog;    cAMP;    morphogenesis;    Gpr161;    Gli repressor;    Mouse;   

DOI  :  10.7554/eLife.67121

来源: eLife Sciences Publications, Ltd

【 摘 要 】

The role of compartmentalized signaling in primary cilia during tissue morphogenesis is not well understood. The cilia localized G protein-coupled receptor, Gpr161, represses hedgehog pathway via cAMP signaling. We engineered a knock-in at the Gpr161 locus in mice to generate a variant (Gpr161mut1), which was ciliary localization defective but cAMP signaling competent. Tissue phenotypes from hedgehog signaling depend on downstream bifunctional Gli transcriptional factors functioning as activators or repressors. Compared to knockout (ko), Gpr161mut1/ko had delayed embryonic lethality, moderately increased hedgehog targets, and partially down-regulated Gli3 repressor. Unlike ko, the Gpr161mut1/ko neural tube did not show Gli2 activator-dependent expansion of ventral-most progenitors. Instead, the intermediate neural tube showed progenitor expansion that depends on loss of Gli3 repressor. Increased extraciliary receptor levels in Gpr161mut1/mut1 prevented ventralization. Morphogenesis in limb buds and midface requires Gli repressor; these tissues in Gpr161mut1/mut1 manifested hedgehog hyperactivation phenotypes—polydactyly and midfacial widening. Thus, ciliary and extraciliary Gpr161 pools likely establish tissue-specific Gli repressor thresholds in determining morpho-phenotypic outcomes.

【 授权许可】

CC BY