Crosstalk between the chloroplast protein import and SUMO systems revealed through genetic and molecular investigation in Arabidopsis

eLife

Na Li¹  Samuel James Watson¹  R Paul Jarvis²  Qihua Ling³  Yiting Ye⁴  Feijie Wu⁵ 

[1] Department of Plant Sciences, University of Oxford, Oxford, United Kingdom;Department of Plant Sciences, University of Oxford, Oxford, United Kingdom;Department of Biology, University of Leicester, Leicester, United Kingdom;Department of Plant Sciences, University of Oxford, Oxford, United Kingdom;National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China;Department of Biology, University of Leicester, Leicester, United Kingdom;National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China;National Key Laboratory of Plant Molecular Genetics, CAS Centre for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China;Department of Biology, University of Leicester, Leicester, United Kingdom;

关键词: chloroplast;    SUMO;    protein translocation;    TOC proteins;    chloroplast protein import;    chloroplast biogenesis;    A. thaliana;   

DOI  :  10.7554/eLife.60960

来源: eLife Sciences Publications, Ltd

【 摘 要 】

The chloroplast proteome contains thousands of different proteins that are encoded by the nuclear genome. These proteins are imported into the chloroplast via the action of the TOC translocase and associated downstream systems. Our recent work has revealed that the stability of the TOC complex is dynamically regulated by the ubiquitin-dependent chloroplast-associated protein degradation pathway. Here, we demonstrate that the TOC complex is also regulated by the small ubiquitin-like modifier (SUMO) system. Arabidopsis mutants representing almost the entire SUMO conjugation pathway can partially suppress the phenotype of ppi1, a pale-yellow mutant lacking the Toc33 protein. This suppression is linked to increased abundance of TOC proteins and improvements in chloroplast development. Moreover, data from molecular and biochemical experiments support a model in which the SUMO system directly regulates TOC protein stability. Thus, we have identified a regulatory link between the SUMO system and the chloroplast protein import machinery.

【 授权许可】

CC BY