Prenatal risk factors and neonatal DNA methylation in very preterm infants

Clinical Epigenetics

Elisabeth C. McGowan¹  Lynne M. Smith²  Steven L. Pastyrnak³  Charles R. Neal⁴  Julie A. Hofheimer⁵  T. Michael O’Shea⁵  Jennifer Check⁶  Jennifer B. Helderman⁶  Sheri A. DellaGrotta⁷  Lynne M. Dansereau⁷  Brian S. Carter⁸  Marie Camerota⁹  Barry M. Lester^1,10  Stefan Graw^1,11  Carmen J. Marsit^1,11  Todd M. Everson^1,12 

[1] Department of Pediatrics, Alpert Medical School of Brown University, Providence, RI, USA;Department of Pediatrics, Harbor-UCLA Medical Center, Torrance, CA, USA;Department of Pediatrics, Spectrum Health-Helen DeVos Hospital, Grand Rapids, MI, USA;Department of Pediatrics, University of Hawaii John A. Burns School of Medicine, Honolulu, HI, USA;Department of Pediatrics, University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, NC, USA;Department of Pediatrics, Wake Forest School of Medicine, Winston-Salem, NC, USA;Department of Pediatrics, Women and Infants Hospital of Rhode Island, 101 Dudley Street, 02905, Providence, RI, USA;Department of Pediatrics-Neonatology, Children’s Mercy Hospital, Kansas City, MO, USA;Department of Psychiatry and Human Behavior, Alpert Medical School of Brown University, Providence, RI, USA;Department of Pediatrics, Women and Infants Hospital of Rhode Island, 101 Dudley Street, 02905, Providence, RI, USA;Department of Psychiatry and Human Behavior, Alpert Medical School of Brown University, Providence, RI, USA;Department of Pediatrics, Women and Infants Hospital of Rhode Island, 101 Dudley Street, 02905, Providence, RI, USA;Department of Pediatrics, Alpert Medical School of Brown University, Providence, RI, USA;Gangarosa Department of Environmental Health, Emory University Rollins School of Public Health, Atlanta, GA, USA;Gangarosa Department of Environmental Health, Emory University Rollins School of Public Health, Atlanta, GA, USA;Department of Epidemiology, Emory University Rollins School of Public Health, Atlanta, GA, USA;

关键词: Prenatal;    Methylation;    Epigenetics;    Epigenome-wide association study (EWAS);    Neonatal;    Preterm;    Buccal;   

DOI  :  10.1186/s13148-021-01164-9

来源: Springer

【 摘 要 】

BackgroundPrenatal risk factors are related to poor health and developmental outcomes for infants, potentially via epigenetic mechanisms. We tested associations between person-centered prenatal risk profiles, cumulative prenatal risk models, and epigenome-wide DNA methylation (DNAm) in very preterm neonates.MethodsWe studied 542 infants from a multi-center study of infants born < 30 weeks postmenstrual age. We assessed 24 prenatal risk factors via maternal report and medical record review. Latent class analysis was used to define prenatal risk profiles. DNAm was quantified from neonatal buccal cells using the Illumina MethylationEPIC Beadarray.ResultsWe identified three latent profiles of women: a group with few risk factors (61%) and groups with elevated physical (26%) and psychological (13%) risk factors. Neonates born to women in higher risk subgroups had differential DNAm at 2 CpG sites. Higher cumulative prenatal risk was associated with methylation at 15 CpG sites, 12 of which were located in genes previously linked to physical and mental health and neurodevelopment.ConclusionWe observed associations between prenatal risk factors and DNAm in very preterm infants using both person-centered and cumulative risk approaches. Epigenetics offers a potential biological indicator of prenatal risk exposure.

【 授权许可】

CC BY