Gut microbiota from green tea polyphenol-dosed mice improves intestinal epithelial homeostasis and ameliorates experimental colitis

Microbiome

Jiangchao Zhao¹  Guolong Zhang²  Bing Zhang³  Dandan Han⁴  Jiaman Pang⁴  Zhenhua Wu⁴  Shilan Wang⁴  Tiantian Li⁴  Na Li⁴  Shimeng Huang⁴  Junjun Wang⁴  Shiyi Zhang⁴  Zhenjiang Zech Xu⁵ 

[1] Department of Animal Science, Division of Agriculture, University of Arkansas, 72701, Fayetteville, AR, USA;Department of Animal and Food Sciences, Oklahoma State University, 74078, Stillwater, OK, USA;Key Laboratory of Animal Epidemiology of the Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, 100193, Beijing, China;State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China;State Key Laboratory of Food Science and Technology, Nanchang University, 214122, Nanchang, China;

关键词: Green tea polyphenol;    Colitis;    Gut microbiota;    Fecal microbiota transplantation;    Sterile fecal filtrate;   

DOI  :  10.1186/s40168-021-01115-9

来源: Springer

【 摘 要 】

BackgroundAlteration of the gut microbiota may contribute to the development of inflammatory bowel disease (IBD). Epigallocatechin-3-gallate (EGCG), a major bioactive constituent of green tea, is known to be beneficial in IBD alleviation. However, it is unclear whether the gut microbiota exerts an effect when EGCG attenuates IBD.ResultsWe first explored the effect of oral or rectal EGCG delivery on the DSS-induced murine colitis. Our results revealed that anti-inflammatory effect and colonic barrier integrity were enhanced by oral, but not rectal, EGCG. We observed a distinct EGCG-mediated alteration in the gut microbiome by increasing Akkermansia abundance and butyrate production. Next, we demonstrated that the EGCG pre-supplementation induced similar beneficial outcomes to oral EGCG administration. Prophylactic EGCG attenuated colitis and significantly enriched short-chain fatty acids (SCFAs)-producing bacteria such as Akkermansia and SCFAs production in DSS-induced mice. To validate these discoveries, we performed fecal microbiota transplantation (FMT) and sterile fecal filtrate (SFF) to inoculate DSS-treated mice. Microbiota from EGCG-dosed mice alleviated the colitis over microbiota from control mice and SFF shown by superiorly anti-inflammatory effect and colonic barrier integrity, and also enriched bacteria such as Akkermansia and SCFAs. Collectively, the attenuation of colitis by oral EGCG suggests an intimate involvement of SCFAs-producing bacteria Akkermansia, and SCFAs, which was further demonstrated by prophylaxis and FMT.ConclusionsThis study provides the first data indicating that oral EGCG ameliorated the colonic inflammation in a gut microbiota-dependent manner. Our findings provide novel insights into EGCG-mediated remission of IBD and EGCG as a potential modulator for gut microbiota to prevent and treat IBD.1weu4UfF6nDLmdPiHVjMmdVideo Abstract

【 授权许可】

CC BY