Transformation of sugarcane molasses into fructooligosaccharides with enhanced prebiotic activity using whole-cell biocatalysts from Aureobasidium pullulans FRR 5284 and an invertase-deficient Saccharomyces cerevisiae 1403-7A

Bioresources and Bioprocessing

Mark D. Harrison¹  Robert E. Speight²  Most Sheauly Khatun³  Morteza Hassanpour³  Ian M. O’Hara⁴  Zhanying Zhang⁴  Solange I. Mussatto⁵ 

[1] Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, 4000, Brisbane, QLD, Australia;School of Biology and Environmental Science, Faculty of Science, Queensland University of Technology, 4000, Brisbane, QLD, Australia;Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, 4000, Brisbane, QLD, Australia;School of Biology and Environmental Science, Faculty of Science, Queensland University of Technology, 4000, Brisbane, QLD, Australia;ARC Centre of Excellence in Synthetic Biology, QUT, 4000, Brisbane, QLD, Australia;Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, 4000, Brisbane, QLD, Australia;School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, 4000, Brisbane, QLD, Australia;Centre for Agriculture and the Bioeconomy, Faculty of Science, Queensland University of Technology, 4000, Brisbane, QLD, Australia;School of Mechanical, Medical and Process Engineering, Faculty of Engineering, Queensland University of Technology, 4000, Brisbane, QLD, Australia;ARC Centre of Excellence in Synthetic Biology, QUT, 4000, Brisbane, QLD, Australia;Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, Building 223, 2800, Kongens Lyngby, Denmark;

关键词: Sugarcane molasses;    A. pullulans;    Transfructosylating activity;    Fructooligosaccharides;    Prebiotics;    Probiotics;   

DOI  :  10.1186/s40643-021-00438-7

来源: Springer

【 摘 要 】

Fructooligosaccharides (FOS) can be used as feed prebiotics, but are limited by high production costs. In this study, low-cost sugarcane molasses was used to produce whole-cell biocatalysts containing transfructosylating enzymes by Aureobasidium pullulans FRR 5284, followed by FOS production from molasses using the whole-cells of A. pullulans. A. pullulans in molasses-based medium produced cells and broth with a total transfructosylating activity of 123.6 U/mL compared to 61.0 and 85.8 U/mL in synthetic molasses-based and sucrose-based media, respectively. It was found that inclusion of glucose in sucrose medium reduced both transfructosylating and hydrolytic activities of the produced cells and broth. With the use of pure glucose medium, cells and broth had very low levels of transfructosylating activities and hydrolytic activities were not detected. These results indicated that A. pullulans FRR 5284 produced both constitutive and inducible enzymes in sucrose-rich media, such as molasses while it only produced constitutive enzymes in the glucose media. Furthermore, treatment of FOS solutions generated from sucrose-rich solutions using an invertase-deficient Saccharomyces yeast converted glucose to ethanol and acetic acid and improved FOS content in total sugars by 20–30%. Treated FOS derived from molasses improved the in vitro growth of nine probiotic strains by 9–63% compared to a commercial FOS in 12 h incubation. This study demonstrated the potential of using molasses to produce FOS for feed application.

【 授权许可】

CC BY