Apocynin ameliorates cognitive deficits in streptozotocin-induced diabetic rats

Bratislava Medical Journal

article

L. Ming¹  P. Huan¹  D. Beiwan¹  L. Kang¹  L. Xianchu³ 

[1] College of Life Science, Hunan University of Arts and Science;Key Laboratory of Physical Fitness and Exercise Rehabilitation of Hunan Province, Hunan Normal University;Department of Hematology, Xiangya Changde Hospital

关键词: DACD;    apocynin;    oxidation;    infl ammation;    apoptosis.;   

DOI  :  10.4149/BLL_2021_010

学科分类：医学（综合）

来源: AEPress, s.r.o.

【 摘 要 】

AIMS: The aim was to investigate the improvement properties of apocynin and its potential mechanism on diabetes-associated cognitive decline. METHODS: In this study, the model of diabetic rat was established by STZ (50 mg/kg) and treated with apocynin (16 mg/kg/d for 12 weeks). The cognitive ability was evaluated by Morris water maze test. The indicators of oxidative stress (SOD and MDA) were analyzed by spectrophotometer. The infl ammatory cytokines were measured by real time-PCR and ELISA. The protein expressions of Nrf-2, HO-1, Bcl-2 and Bax were determined by Western blot. RESULTS: Treatment with apocynin ameliorated diabetes-related learning and memory injury, as represented by decreasing escape latency and enhancement of the number of times of crossing platform, in the Morris water maze test. In hippocampus, apocynin markedly augmented SOD activity and inhibited MDA level to alleviate oxidative stress. Moreover, apocynin obviously relieved infl ammatory reaction by suppressing TNF-α, IL-1β and IL-6 concentrations. Concomitantly, apocynin also statistically enhanced Nrf-2 and HO-1 protein expression to improve DACD. Lastly, apocynin notably ameliorated Bax/Bcl-2 ratio by regulating Bax and Bcl2 protein expression to mitigate apoptosis.

【 授权许可】

CC BY