Mesenchymal Stromal Cells Suppress Hippocampal Neuron Autophagy Stress Induced by Hypoxic-Ischemic Brain Damage: The Possible Role of Endogenous IL-6 Secretion

Neural Plasticity

Lu Xiao¹  Ting Yang¹  Xiaohua Liang¹  Jie Chen¹  Miao Yang¹  Mulan He¹  Yan Gu¹  Wuqing Sun² 

[1] Children’s Nutrition Research Center, Children’s Hospital of Chongqing Medical University, Chongqing 400014, China, cqmu.edu.cn;Chongqing Key Laboratory of Child Nutrition and Health, Chongqing 400014, China;Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing 400014, China, moe.edu.cn;China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing 400014, China;Chongqing Key Laboratory of Child Nutrition and Health, Chongqing 400014, China;Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing 400014, China, moe.edu.cn;China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing 400014, China;Information Technological Service Center, Children’s Hospital of Chongqing Medical University, Chongqing 400014, China, cqmu.edu.cn;

DOI  :  10.1155/2020/8822579

来源: Hindawi Publishing Corporation

【 摘 要 】

Background. Increasing evidence has revealed that mesenchymal stromal cell (MSC) transplantation alleviates hypoxic-ischemic brain damage (HIBD) induced neurological impairments via immunomodulating astrocyte antiapoptosis effects. However, it remains unclear whether MSCs regulate neuron autophagy following HIBD. Results. In the present study, MSC transplantation effectively ameliorated learning-memory function and suppressed stress-induced hippocampal neuron autophagy in HIBD rats. Moreover, the suppressive effects of MSCs on autophagy were significantly weakened following endogenous IL-6 silencing in MSCs. Suppressing IL-6 expression also significantly increased p-AMPK protein expression and decreased p-mTOR protein expression in injured hippocampal neurons. Conclusion. Endogenous IL-6 in MSCs may reduce autophagy in hippocampal neurons partly through the AMPK/mTOR pathway.

【 授权许可】

Unknown