A novel conjunctive microenvironment derived from human subcutaneous adipose tissue contributes to physiology of its superficial layer

Stem Cell Research & Therapy

Celine Auxenfans¹  Lara Jobeili¹  Isis Côrtes²  Leandra Santos Baptista³  Bianca Montenegro⁴  Cesar Claudio-da-Silva⁵  Marielle Bouschbacher⁶  Dominique Sigaudo-Roussel⁷ 

[1] Hospital Civilian of Lyon, Hospital Edouard Herriot, 69003, Lyon, France;Univ Lyon, Université Claude Bernard Lyon 1, CNRS, LBTI UMR5305, 69367, Lyon, France;Multidisciplinary Center for Biological Research (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerém, 25245-390, Duque de Caxias, Rio de Janeiro, Brazil;Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 25250-020, Duque de Caxias, Rio de Janeiro, Brazil;Post-Graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 25250-020, Duque de Caxias, Rio de Janeiro, Brazil;Multidisciplinary Center for Biological Research (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerém, 25245-390, Duque de Caxias, Rio de Janeiro, Brazil;Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 25250-020, Duque de Caxias, Rio de Janeiro, Brazil;Post-Graduation Program in Biotechnology, National Institute of Metrology, Quality and Technology (Inmetro), 25250-020, Duque de Caxias, Rio de Janeiro, Brazil;Post-Graduation Program of Translational Biomedicine (Biotrans), Unigranrio, Campus I,, 25071-202, Duque de Caxias, Rio de Janeiro, Brazil;Multidisciplinary Center for Biological Research (Numpex-Bio), Federal University of Rio de Janeiro (UFRJ) Xerém, 25245-390, Duque de Caxias, Rio de Janeiro, Brazil;Laboratory of Tissue Bioengineering, National Institute of Metrology, Quality and Technology (Inmetro), 25250-020, Duque de Caxias, Rio de Janeiro, Brazil;Post-Graduation Program of Translational Biomedicine (Biotrans), Unigranrio, Campus I,, 25071-202, Duque de Caxias, Rio de Janeiro, Brazil;Plastic Surgery Service, Clementino Fraga Filho University Hospital (HUCFF), Federal University of Rio de Janeiro (UFRJ), 21941-913, Rio de Janeiro, Rio de Janeiro, Brazil;URGO Research Innovation and Development, Chenôve, France;Univ Lyon, Université Claude Bernard Lyon 1, CNRS, LBTI UMR5305, 69367, Lyon, France;

关键词: Human subcutaneous adipose tissue;    Superficial microenvironment;    Deeper microenvironment;    Retinacula cutis microenvironment;    Stromal vascular fraction;    Adipose stromal/stem cells;   

DOI  :  10.1186/s13287-021-02554-9

来源: Springer

【 摘 要 】

BackgroundIn human subcutaneous adipose tissue, the superficial fascia distinguishes superficial and deep microenvironments showing extensions called retinacula cutis. The superficial subcutaneous adipose tissue has been described as hyperplastic and the deep subcutaneous adipose tissue as inflammatory. However, few studies have described stromal-vascular fraction (SVF) content and adipose-derived stromal/stem cells (ASCs) behavior derived from superficial and deep subcutaneous adipose tissue. In this study, we analyzed a third conjunctive microenvironment: the retinacula cutis superficialis derived from superficial subcutaneous adipose tissue.MethodsThe samples of abdominal human subcutaneous adipose tissue were obtained during plastic aesthetic surgery in France (Declaration DC-2008-162) and Brazil (Protocol 145/09).ResultsThe SVF content was characterized in situ by immunofluorescence and ex vivo by flow cytometry revealing a high content of pre-adipocytes rather in superficial subcutaneous adipose tissue microenvironment. Adipogenic assays revealed higher percentage of lipid accumulation area in ASCs from superficial subcutaneous adipose tissue compared with retinacula cutis superficialis (p < 0.0001) and deep subcutaneous adipose tissue (p < 0.0001). The high adipogenic potential of superficial subcutaneous adipose tissue was corroborated by an up-regulation of adipocyte fatty acid-binding protein (FABP4) compared with retinacula cutis superficialis (p < 0.0001) and deep subcutaneous adipose tissue (p < 0.0001) and of C/EBPα (CCAAT/enhancer-binding protein alpha) compared with retinacula cutis superficialis (p < 0.0001) and deep subcutaneous adipose tissue (p < 0.0001) microenvironments. Curiously, ASCs from retinacula cutis superficialis showed a higher level of adiponectin receptor gene compared with superficial subcutaneous adipose tissue (p = 0.0409), widely known as an anti-inflammatory hormone. Non-induced ASCs from retinacula cutis superficialis showed higher secretion of human vascular endothelial growth factor (VEGF), compared with superficial (p = 0.0485) and deep (p = 0.0112) subcutaneous adipose tissue and with adipogenic-induced ASCs from superficial (p = 0.0175) and deep (p = 0.0328) subcutaneous adipose tissue. Furthermore, ASCs from retinacula cutis superficialis showed higher secretion of Chemokine (C–C motif) ligand 5 (CCL5) compared with non-induced (p = 0.0029) and induced (p = 0.0089) superficial subcutaneous adipose tissue.ConclusionsThis study highlights the contribution to ASCs from retinacula cutis superficialis in their angiogenic property previously described for the whole superficial subcutaneous adipose tissue besides supporting its adipogenic potential for superficial subcutaneous adipose tissue.

【 授权许可】

CC BY