α-Farnesene production from lipid by engineered Yarrowia lipolytica

Bioresources and Bioprocessing

Zhiyong Cui¹  Jin Hou¹  Yinghang Liu¹  Zhaoxuan Wang¹  Qingsheng Qi² 

[1] State Key Laboratory of Microbial Technology, Shandong University, Binhai Road 72, 266237, Qingdao, People’s Republic of China;State Key Laboratory of Microbial Technology, Shandong University, Binhai Road 72, 266237, Qingdao, People’s Republic of China;CAS Key Lab of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 266101, Qingdao, People’s Republic of China;

关键词: α-Farnesene;    Erg12;    Oleic acid;    Waste cooking oils;    Yarrowia lipolytica;   

DOI  :  10.1186/s40643-021-00431-0

来源: Springer

【 摘 要 】

Producing high value-added products from waste lipid feedstock by microbial cell factory has great advantages to minimize the pollution as well as improve the economic value of wasted oils and fats. Yarrowia lipolytica is a non-conventional oleaginous yeast and can grow on a variety of hydrophobic substrates. In this study, we explored its ability to synthesize α-farnesene, an important sesquiterpene, using lipid feedstock. Based on the α-farnesene production strain, we constructed previously, we identified that Erg12 was the key limiting factor to further increase the α-farnesene production. The α-farnesene production was improved by 35.8% through increasing the copy number of ERG12 and FSERG20 on oleic acid substrate. Expression of heterologous VHb further improved α-farnesene production by 12.7%. Combining metabolic engineering with the optimization of fermentation conditions, the α-farnesene titer and yield reached 10.2 g/L and 0.1 g/g oleic acid, respectively, in fed-batch cultivation. The α-farnesene synthesis ability on waste cooking oil and other edible oils were also explored. Compared with using glucose as carbon source, using lipid substrates obtained higher α-farnesene yield and titer, but lower by-products accumulation, demonstrating the advantage of Y. lipolytica to synthesize high value-added products using lipid feedstock.

【 授权许可】

CC BY