Conductive single-wall carbon nanotubes/extracellular matrix hybrid hydrogels promote the lineage-specific development of seeding cells for tissue repair through reconstructing an integrin-dependent niche

Journal of Nanobiotechnology

Xiaoming Zhu¹  Chao Yuan¹  Xiaoyu Ding¹  Jinmiao Shi¹  Bingshui Xiu¹  Zhiqiang Liu¹  Zengqiang Yuan¹  Jiao Zhang²  Jianfeng Liu³  Rui Bai⁴  Zhigeng Jin⁴  Huiliang Liu⁴  Yi Li⁴ 

[1] Beijing Institute of Basic Medical Sciences, 100850, Beijing, China;Department of Cardiology, Beijing Electric Power Hospital, State Grid Corporation of China, 100073, Beijing, China;Department of Cardiology, The Second Medical Center & National Clinical Research Center for Geriatric Diseases, Chinese PLA General Hospital, 100853, Beijing, China;Senior Department of Cardiology, The Sixth Medical Center of PLA General Hospital, 100048, Beijing, China;

关键词: Hybrid hydrogel;    Single-wall carbon nanotubes;    Extracellular matrixes;    Regenerative medicine;    Bioactive scaffolds;   

DOI  :  10.1186/s12951-021-00993-3

来源: Springer

【 摘 要 】

BackgroundThe niche of tissue development in vivo involves the growth matrix, biophysical cues and cell-cell interactions. Although natural extracellular matrixes may provide good supporting for seeding cells in vitro, it is evitable to destroy biophysical cues during decellularization. Reconstructing the bioactivities of extracellular matrix-based scaffolds is essential for their usage in tissue repair.ResultsIn the study, a hybrid hydrogel was developed by incorporating single-wall carbon nanotubes (SWCNTs) into heart-derived extracellular matrixes. Interestingly, insoluble SWCNTs were well dispersed in hybrid hydrogel solution via the interaction with extracellular matrix proteins. Importantly, an augmented integrin-dependent niche was reconstructed in the hybrid hydrogel, which could work like biophysical cues to activate integrin-related pathway of seeding cells. As supporting scaffolds in vitro, the hybrid hydrogels were observed to significantly promote seeding cell adhesion, differentiation, as well as structural and functional development towards mature cardiac tissues. As injectable carrier scaffolds in vivo, the hybrid hydrogels were then used to delivery stem cells for myocardial repair in rats. Similarly, significantly enhanced cardiac differentiation and maturation(12.5 ± 2.3% VS 32.8 ± 5%) of stem cells were detected in vivo, resulting in improved myocardial regeneration and repair.ConclusionsThe study represented a simple and powerful approach for exploring bioactive scaffold to promote stem cell-based tissue repair.Graphic abstract

【 授权许可】

CC BY