Characterization of three glutamate decarboxylases from Bacillus spp. for efficient γ-aminobutyric acid production

Microbial Cell Factories

Cheng Zhou¹  Xiu Zhang¹  Bin Yao²  Yuan Wang²  Tao Tu²  Huiying Luo²  Xiaoyun Su²  Lei Sun²  Yingguo Bai²  Jie Zhang² 

[1] Ningxia Key Laboratory for the Development and Application of Microbial Resources in Extreme Environments, North Minzu University, 750021, Yinchuan, China;State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, 100193, Beijing, China;

关键词: γ-Aminobutyric acid;    Bioconversion;    Glutamate decarboxylase;    Biocatalyst;   

DOI  :  10.1186/s12934-021-01646-8

来源: Springer

【 摘 要 】

BackgroundGamma-aminobutyric acid (GABA) is an important bio-product used in pharmaceuticals and functional foods and as a precursor of the biodegradable plastic polyamide 4. Glutamate decarboxylase (GAD) converts l-glutamate (l-Glu) into GABA via decarboxylation. Compared with other methods, develop a bioconversion platform to produce GABA is of considerable interest for industrial use.ResultsThree GAD genes were identified from three Bacillus strains and heterologously expressed in Escherichia coli BL21 (DE3). The optimal reaction temperature and pH values for three enzymes were 40 °C and 5.0, respectively. Of the GADs, GADZ11 had the highest catalytic efficiency towards l-Glu (2.19 mM− 1 s− 1). The engineered E. coli strain that expressed GADZ11 was used as a whole-cell biocatalyst for the production of GABA. After repeated use 14 times, the cells produced GABA with an average molar conversion rate of 98.6% within 14 h.ConclusionsThree recombinant GADs from Bacillus strains have been conducted functional identification. The engineered E. coli strain heterologous expressing GADZ1, GADZ11, and GADZ20 could accomplish the biosynthesis of l-Glu to GABA in a buffer-free reaction at a high l-Glu concentration. The novel engineered E. coli strain has the potential to be a cost-effective biotransformation platform for the industrial production of GABA.

【 授权许可】

CC BY