Genetic mapping high protein content QTL from soybean ‘Nanxiadou 25’ and candidate gene analysis

BMC Plant Biology

Zhaoqiong Zeng¹  Jun Feng¹  Haiying Wu¹  Mingrong Zhang¹  Jianqiu Lian¹  Jiangang An¹  Xiaobo Yu¹  Wenying Yang¹  Jia Wang²  Liezhao Liu³  Lin Mao³ 

[1] Nanchong Academy of Agricultural Sciences, 637000, Nanchong, Sichuan, China;Nanchong Academy of Agricultural Sciences, 637000, Nanchong, Sichuan, China;Southwest University, 400715, Chongqing, China;Southwest University, 400715, Chongqing, China;

关键词: Soybean;    Seed protein content;    Quantitative trait loci (QTL);    QTL-seq;    RNA-seq;   

DOI  :  10.1186/s12870-021-03176-2

来源: Springer

【 摘 要 】

BackgroundSoybean is a globally important legume crop that provides a primary source of high-quality vegetable protein and oil. Seed protein content (SPC) is a valuable quality trait controlled by multiple genes in soybean.ResultsIn this study, we performed quantitative trait loci (QTL) mapping, QTL-seq, and RNA sequencing (RNA-seq) to reveal the genes controlling protein content in the soybean by using the high protein content variety Nanxiadou 25. A total of 50 QTL for SPC distributed on 14 chromosomes except chromosomes 4, 12, 14, 17, 18, and 19 were identified by QTL mapping using 178 recombinant inbred lines (RILs). Among these QTL, the major QTL qSPC_20–1 and qSPC_20–2 on chromosome 20 were repeatedly detected across six tested environments, corresponding to the location of the major QTL detected using whole-genome sequencing-based QTL-seq. 329 candidate DEGs were obtained within the QTL region of qSPC_20–1 and qSPC_20–2 via gene expression profile analysis. Nine of which were associated with SPC, potentially representing candidate genes. Clone sequencing results showed that different single nucleotide polymorphisms (SNPs) and indels between high and low protein genotypes in Glyma.20G088000 and Glyma.16G066600 may be the cause of changes in this trait.ConclusionsThese results provide the basis for research on candidate genes and marker-assisted selection (MAS) in soybean breeding for seed protein content.

【 授权许可】

CC BY