rAAV9‐mediated supplementation of miR-29b improve angiotensin-II induced renal fibrosis in mice

Molecular Medicine

Ju-hong Zhang¹  Jing Li¹  Yang Ye¹  Wang-qi Yu² 

[1] Department of Cardiology, Sir Run Run Shaw Hospital, Zhejiang University, 310016, Hangzhou, Zhejiang, People’s Republic of China;The Affiliated Hospital of Hangzhou Normal University, No. 1 Wenzhou Road, Gong Shu District, 310016, Hangzhou, Zhejiang, People’s Republic of China;

关键词: Collagen;    Extracellular matrix deposition;    Recombinant adeno-associated virus;    Renal interstitial fibrosis;    miR-29b;    Gene delivery;   

DOI  :  10.1186/s10020-021-00349-5

来源: Springer

【 摘 要 】

BackgroundRenin–angiotensin–aldosterone system activation is the critical factor in renal remodeling and dysfunction. Our previous study suggested that miR-29b may attenuate AngII-induced renal intestinal fibrosis in vitro. In the present study, we aimed to determine whether recombinant rAAV9-mediated miR-29b delivery protects against AngII-induced renal fibrosis and dysfunction.MethodMice were treated with AngII via osmotic mini-pumps, or phosphate-buffered saline. rAAV9 vectors were produced using the rBac-based system in SF9 cells. rAAV9-miR-29b or rAAV9-control-miR was injected into the kidneys of mice subjected to the model of AngII infusion. The role of miR-29b in renal fibrosis was assessed using quantitative polymerase chain reaction, western blot, and histology.ResultsIn AngII-induced fibrotic kidney tissue, miR-29b expression was downregulated. rAAV9-miR-29b delivery significantly reversed renal injury as indicated by decreased serum creatinine and injury related gene expression in AngII-infused mice. Regarding organ remodeling, tubulointerstitial fibrosis and deposition of extracellular matrix components such as collagen type I and type III were significantly decreased in renal tissue from mice delivered rAAV9-miR-29b.ConclusionOur results demonstrate great potential for use of rAAV9 as an applicable vector for delivery of miR-29b as an antifibrogenic factor for treatment of tubulointerstitial fibrosis-induced renal injury.

【 授权许可】

CC BY