Donkey-derived anti-CDV IgG, as a passive immunotherapy agent, can effectively increase survival rates of the experimental CDV-infected dogs

BMC Veterinary Research

Nan Li¹  Lanhui Li¹  Fengyang Wu¹  Yueqiang Guan²  Dan Cui³  Yuzhu Zuo³  Jianlou Zhang³  Yonghong Zhang³  Shanshan Huo³  Fei Zhong³  Zhiqiang Zheng³  Wenyan Li⁴ 

[1] School of Animal Science and Technology, Hebei Agricultural University, 289 Lingyusi Streat, 071001, Baoding, Hebei, China;School of Life Science, Hebei University, 180 Wusi East Road, 071002, Baoding, Hebei, China;School of Veterinary Medicine, Hebei Veterinary Biotechnology Innovation Center, Hebei Agricultural University, 289 Lingyusi Streat, 071001, Baoding, Hebei, China;School of Veterinary Medicine, Hebei Veterinary Biotechnology Innovation Center, Hebei Agricultural University, 289 Lingyusi Streat, 071001, Baoding, Hebei, China;School of Basic Medicine, Hebei University, 342 Yuhua East Road, 071002, Baoding, Hebei, China;

关键词: Canine distemper virus;    Passive immunotherapy;    Heterologous antibody;    Donkey;    Therapeutic effects;   

DOI  :  10.1186/s12917-021-02982-y

来源: Springer

【 摘 要 】

BackgroundHumoral immunity plays an important role in the prevention of canine distemper. Anti-CD virus (CDV) antibody has strong antiviral activity and is widely used in the treatment of CD. However, with the increase of CD cases, the availability of therapeutic CD antibody fell short of the clinical needs.ResultsThe high-titer antiserum with the high-titer neutralizing activity against CDV was obtained from the donkeys (Dezhou Donkey) immunized with the inactivated CDV vaccine. The donkey anti-CDV IgG was purified from the donkey serum, which was identified to significantly inhibit the CDV replication in the cultured Vero cells and effectively reduce the clinical symptoms and increase the survival rates (75%) of CDV-infected dogs (Shih-tzu Dog), similar to that treated with the dog-derived anti-CDV IgG. These results indicate that donkey-derived IgG is a potential substitute for dog-derived IgG to treat the CD in clinic.ConclusionsAdministration of donkey-derived anti-CDV IgG can ameliorate clinical symptoms and inhibit virus replication, thereby increasing the survival of CDV-infected dogs. This study opens up a new source of therapeutic antibody for CD treatment.

【 授权许可】

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