Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression

Human Genomics

Zi-Wei Du¹  Fan Meng¹  Lu-Lu Wei¹  Ya-Ping Zhou¹  Muhammad Naveed¹  Jing Zhang¹  Lian-Di Li¹  Qi-Gang Zhou²  Feng Han³  Chun Wang⁴  Huachen Ding⁴  Kai Gu⁵ 

[1] Department of Clinical Pharmacology, School of Pharmacy, Nanjing Medical University, 211166, Nanjing, Jiangsu Province, China;Department of Clinical Pharmacology, School of Pharmacy, Nanjing Medical University, 211166, Nanjing, Jiangsu Province, China;Sir Run Run Hospital, Nanjing Medical University, 211167, Nanjing, Jiangsu Province, China;Key Laboratory of Cardiovascular and Cerebrovascular Medicine, School of Pharmacy, Nanjing Medical University, 211166, Nanjing, Jiangsu Province, China;Nanjing Brain Hospital Affiliated to Nanjing Medical University, 210029, Nanjing, Jiangsu Province, China;Functional Brain Imaging Institute of Nanjing Medical University, 210029, Nanjing, Jiangsu Province, China;Sir Run Run Hospital, Nanjing Medical University, 211167, Nanjing, Jiangsu Province, China;

关键词: Depression;    Exosomes;    MicroRNAs;    Biomarker;    High-throughput sequencing;   

DOI  :  10.1186/s40246-021-00354-z

来源: Springer

【 摘 要 】

Whether microRNAs (miRNAs) from plasma exosomes might be dysregulated in patients with depression, especially treatment-resistant depression (TRD), remains unclear, based on study of which novel biomarkers and therapeutic targets could be discovered. To this end, a small sample study was performed by isolation of plasma exosomes from patients with TRD diagnosed by Hamilton scale. In this study, 4 peripheral plasma samples from patients with TRD and 4 healthy controls were collected for extraction of plasma exosomes. Exosomal miRNAs were analyzed by miRNA sequencing, followed by image collection, expression difference analysis, target gene GO enrichment analysis, and KEGG pathway enrichment analysis. Compared with the healthy controls, 2 miRNAs in the plasma exosomes of patients with TRD showed significant differences in expression, among which has-miR-335-5p were significantly upregulated and has-miR-1292-3p were significantly downregulated. Go and KEGG analysis showed that dysregulated miRNAs affect postsynaptic density and axonogenesis as well as the signaling pathway of axon formation and cell growths. The identification of these miRNAs and their target genes may provide novel biomarkers for improving diagnosis accuracy and treatment effectiveness of TRD.

【 授权许可】

CC BY