Human Genomics | |
Abnormal expression profile of plasma-derived exosomal microRNAs in patients with treatment-resistant depression | |
Zi-Wei Du1  Fan Meng1  Lu-Lu Wei1  Ya-Ping Zhou1  Muhammad Naveed1  Jing Zhang1  Lian-Di Li1  Qi-Gang Zhou2  Feng Han3  Chun Wang4  Huachen Ding4  Kai Gu5  | |
[1] Department of Clinical Pharmacology, School of Pharmacy, Nanjing Medical University, 211166, Nanjing, Jiangsu Province, China;Department of Clinical Pharmacology, School of Pharmacy, Nanjing Medical University, 211166, Nanjing, Jiangsu Province, China;Sir Run Run Hospital, Nanjing Medical University, 211167, Nanjing, Jiangsu Province, China;Key Laboratory of Cardiovascular and Cerebrovascular Medicine, School of Pharmacy, Nanjing Medical University, 211166, Nanjing, Jiangsu Province, China;Nanjing Brain Hospital Affiliated to Nanjing Medical University, 210029, Nanjing, Jiangsu Province, China;Functional Brain Imaging Institute of Nanjing Medical University, 210029, Nanjing, Jiangsu Province, China;Sir Run Run Hospital, Nanjing Medical University, 211167, Nanjing, Jiangsu Province, China; | |
关键词: Depression; Exosomes; MicroRNAs; Biomarker; High-throughput sequencing; | |
DOI : 10.1186/s40246-021-00354-z | |
来源: Springer | |
【 摘 要 】
Whether microRNAs (miRNAs) from plasma exosomes might be dysregulated in patients with depression, especially treatment-resistant depression (TRD), remains unclear, based on study of which novel biomarkers and therapeutic targets could be discovered. To this end, a small sample study was performed by isolation of plasma exosomes from patients with TRD diagnosed by Hamilton scale. In this study, 4 peripheral plasma samples from patients with TRD and 4 healthy controls were collected for extraction of plasma exosomes. Exosomal miRNAs were analyzed by miRNA sequencing, followed by image collection, expression difference analysis, target gene GO enrichment analysis, and KEGG pathway enrichment analysis. Compared with the healthy controls, 2 miRNAs in the plasma exosomes of patients with TRD showed significant differences in expression, among which has-miR-335-5p were significantly upregulated and has-miR-1292-3p were significantly downregulated. Go and KEGG analysis showed that dysregulated miRNAs affect postsynaptic density and axonogenesis as well as the signaling pathway of axon formation and cell growths. The identification of these miRNAs and their target genes may provide novel biomarkers for improving diagnosis accuracy and treatment effectiveness of TRD.
【 授权许可】
CC BY
【 预 览 】
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