CircFAM13B promotes the proliferation of hepatocellular carcinoma by sponging miR-212, upregulating E2F5 expression and activating the P53 pathway

Cancer Cell International

Wensheng Xu¹  Xiucui Zhang¹  Xinghao Cao¹  Junjie Zhan¹  Xiaofeng Hang¹  Yuanjing Zhang¹  Jianrong Wang¹  Junxue Wang¹  Jing Gu²  Jianhe Gan²  Ying Xie³ 

[1] Department of Infectious Disease, Changzheng Hospital, Naval Medical University, 415 Fengyang street, 200003, Shanghai, China;Department of Infectious Disease, The First Affiliated Hospital of Soochow University, 188 Shizi street, 215000, Suzhou, China;Department of Infectious Disease, The First Affiliated Hospital of Soochow University, 188 Shizi street, 215000, Suzhou, China;Department of Infectious Disease, Changzheng Hospital, Naval Medical University, 415 Fengyang street, 200003, Shanghai, China;

关键词: circFAM13B;    miR-212;    E2F5;    P53;    HCC;   

DOI  :  10.1186/s12935-021-02120-6

来源: Springer

【 摘 要 】

BackgroundMost of the biological functions of circular RNAs (circRNAs) and the potential underlying mechanisms in hepatocellular carcinoma (HCC) have not yet been discovered.MethodsIn this study, using circRNA expression data from HCC tumor tissues and adjacent tissues from the Gene Expression Omnibus database, we identified out differentially expressed circRNAs and verified them by qRT-PCT. Functional experiments were performed to evaluate the effects of circFAM13B in HCC in vitro and in vivo.ResultsWe found that circFAM13B was the most significantly differentially expressed circRNA in HCC tissue. Subsequently, in vitro and in vivo studies also demonstrated that circFAM13B promoted the proliferation of HCC. Further studies revealed that circFAM13B, a sponge of miR-212, is involved in the regulation of E2F5 gene expression by competitively binding to miR-212, inhibits the activation of the P53 signalling pathway, and promotes the proliferation of HCC cells.ConclusionsOur findings revealed the mechanism underlying the regulatory role played by circFAM13B, miR-212 and E2F5 in HCC. This study provides a new theoretical basis and novel target for the clinical prevention and treatment of HCC.

【 授权许可】

CC BY