Identification of Brassica napus small RNAs responsive to infection by a necrotrophic pathogen

BMC Plant Biology

Mark C. Derbyshire¹  Toby E. Newman¹  Lars G. Kamphuis²  Roshan Regmi² 

[1] Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, 6102, Bentley, WA, Australia;Centre for Crop and Disease Management, School of Molecular and Life Sciences, Curtin University, 6102, Bentley, WA, Australia;Commonwealth Scientific and Industrial Research Organisation, Agriculture and Food, 6014, Floreat, WA, Australia;

关键词: Small RNA;    micro RNA;    Degradome;    Fungal pathogen;    B. napus;    PHAS locus;    TAS gene;    Ta-siRNA;    Pha-siRNA;   

DOI  :  10.1186/s12870-021-03148-6

来源: Springer

【 摘 要 】

BackgroundSmall RNAs are short non-coding RNAs that are key gene regulators controlling various biological processes in eukaryotes. Plants may regulate discrete sets of sRNAs in response to pathogen attack. Sclerotinia sclerotiorum is an economically important pathogen affecting hundreds of plant species, including the economically important oilseed B. napus. However, there are limited studies on how regulation of sRNAs occurs in the S. sclerotiorum and B. napus pathosystem.ResultsWe identified different classes of sRNAs from B. napus using high throughput sequencing of replicated mock and infected samples at 24 h post-inoculation (HPI). Overall, 3999 sRNA loci were highly expressed, of which 730 were significantly upregulated during infection. These 730 up-regulated sRNAs targeted 64 genes, including disease resistance proteins and transcriptional regulators. A total of 73 conserved miRNA families were identified in our dataset. Degradome sequencing identified 2124 cleaved mRNA products from these miRNAs from combined mock and infected samples. Among these, 50 genes were specific to infection. Altogether, 20 conserved miRNAs were differentially expressed and 8 transcripts were cleaved by the differentially expressed miRNAs miR159, miR5139, and miR390, suggesting they may have a role in the S. sclerotiorum response. A miR1885-triggered disease resistance gene-derived secondary sRNA locus was also identified and verified with degradome sequencing. We also found further evidence for silencing of a plant immunity related ethylene response factor gene by a novel sRNA using 5′-RACE and RT-qPCR.ConclusionsThe findings in this study expand the framework for understanding the molecular mechanisms of the S. sclerotiorum and B. napus pathosystem at the sRNA level.

【 授权许可】

CC BY