Monocytes-based in vitro assay for a preliminary biocompatibility assessment of blood-contacting devices

European Journal of Inflammation

article

Claudia Gemelli¹  Alexis Grande²  Sergio Ferrari²  Aldo Tomasi¹  Aurora Cuoghi¹ 

[1]Science and Technology Park for Medicine

[2]Department of Life Science, University of Modena and Reggio Emilia

[3]Department of Diagnostic and Clinical Medicine and Public Health, University of Modena and Reggio Emilia

关键词: biocompatibility;    biomaterials;    blood-contacting devices;    cell toxicity;    extractable compound;    inflammation;    medical devices;    monocytes;   

DOI  :  10.1177/2058739218820479

学科分类：内科医学

来源: Sage Journals

【 摘 要 】

The biological evaluation of biomaterials is currently defined by the ISO-10993 norm in which parts four and five are dedicated to emo-compatibility and cell toxicity, respectively. Our study will provide a novel in vitro experimental approach for the biocompatibility assessment of biomaterials or medical devices using human primary monocytes as cellular model. In these new settings, human monocytes are exposed to a medium containing the extractable compounds derived from materials or devices; subsequently, cell toxicity and pro-inflammatory effects are analysed through MTT assay, flow cytometry and enzyme-linked immunosorbent assay (ELISA) methodologies. These experimental procedures offer the advantage to use a human and primary cell context belonging to the immune system, in order to accurately predict the nature of blood/device interaction occurring during a clinical application. To validate the reliability of this method, we also reported a comparative study between two different membranes showing a different level of biocompatibility. On the bases of these data, it is possible to state that this new experimental model represents a good approach to investigate the effects induced by a biomaterial on cell death and inflammation using human, primary monocytes.

【 授权许可】

CC BY|CC BY-NC