Immunological pathways of macrophage response to Brucella ovis infection

Innate immunity

article

Zhixiong Zhou¹  Jixuan Chen²  Cailiang Fan¹  Qingzhou Huang¹  Baoru Han⁴  Jianjun Wen⁵  Hanwei Jiao⁶  Guojing Gu¹  Yichen Luo¹  Wenjie Li¹  Bowen Li¹  Yu Zhao¹  Juan Liu¹  Xuehong Shuai¹  Li Wu¹ 

[1] College of Veterinary Medicine, Southwest University;Veterinary Scientific Engineering Research Center;Animal Disease Prevention and Control Center of Rongchang;College of Medical Informatics, Chongqing Medical University;Department of Microbiology and Immunology, University of Texas Medical Branch at Galveston;Immunology Research Center, Medical Research Institute, Southwest University

关键词: Brucella ovis;    macrophage;    immunological pathways;    host;    pathogenic;   

DOI  :  10.1177/1753425920958179

来源: Sage Journals

【 摘 要 】

As the molecular mechanisms of Brucella ovis pathogenicity are not completely clear, we have applied a transcriptome approach to identify the differentially expressed genes (DEGs) in RAW264.7 macrophage infected with B. ovis . The DEGs related to immune pathway were identified by Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) functional enrichment analysis. Quantitative real-time PCR (qRT-PCR) was performed to validate the transcriptome sequencing data. In total, we identified 337 up-regulated and 264 down-regulated DEGs in B. ovis -infected group versus mock group. Top 20 pathways were enriched by KEGG analysis and 20 GO by functional enrichment analysis in DEGs involved in the molecular function, cellular component, and biological process and so on, which revealed multiple immunological pathways in RAW264.7 macrophage cells in response to B. ovis infection, including inflammatory response, immune system process, immune response, cytokine activity, chemotaxis, chemokine-mediated signaling pathway, chemokine activity, and CCR chemokine receptor binding. qRT-PCR results showed Ccl2 (ENSMUST00000000193), Ccl2 (ENSMUST00000124479), Ccl3 (ENSMUST00000001008), Hmox1 (ENSMUST00000005548), Hmox1 (ENSMUST00000159631), Cxcl2 (ENSMUST00000075433), Cxcl2 (ENSMUST00000200681), Cxcl2 (ENSMUST00000200919), and Cxcl2 (ENSMUST00000202317). Our findings firstly elucidate the pathways involved in B. ovis -induced host immune response, which may lay the foundation for revealing the bacteria–host interaction and demonstrating the pathogenic mechanism of B. ovis .

【 授权许可】

CC BY|CC BY-NC