Immunophenotypic, cytotoxic, proteomic and genomic characterization of human cord blood vs. peripheral blood CD56 Dim NK cells

Innate immunity

article

Evan Shereck¹  Nancy S Day²  Aradhana Awasthi³  Janet Ayello³  Yaya Chu³  Catherine McGuinn²  Carmella van de Ven³  Megan S Lim⁴  Mitchell S Cairo³ 

[1] Department of Pediatrics, Oregon Health and Science University;Department of Pediatrics, Columbia University;Department of Pediatrics, New York Medical College;Department of Pathology and Laboratory Medicine, University of Pennsylvania;Department of Medicine, New York Medical College;Department of Pathology, New York Medical College;Department of Microbiology and Immunology, New York Medical College;Department Cell Biology and Anatomy, New York Medical College

关键词: CD56;    cord blood;    genomics;    innate immunity;    NK cells;   

DOI  :  10.1177/1753425919846584

来源: Sage Journals

【 摘 要 】

Unrelated cord blood (CB) is an excellent alternative as an allogeneic donor source for stem cell transplantation. CB transplantation is associated with lower incidence of severe acute graft versus host disease (GVHD) and chronic GVHD but similar rates of malignant relapse compared with other unrelated donor cell transplants. NK cells are critical innate immune components and the comparison of CB vs. peripheral blood (PB) NK cells is relatively unknown. NK cell receptor expression, cell function, and maturation may play a role in the risk of relapse after CB transplant. We investigated CB vs. PB NK cell subset cytotoxicity, function, receptor expression, and genomic and proteomic signatures. The CB CD56 dim compared with PB CD56 dim demonstrated significantly increased expression of NKG2A and NKG2D, respectively. CB vs. PB CD56 dim NK cells had significantly decreased in vitro cytotoxicity against a variety of non-Hodgkin lymphoma targets. Various proteins were significantly under- and over-expressed in CB vs. PB CD56 dim NK cells. Microarray analyses and qRT-PCR in CB vs. PB CD56 dim demonstrated significantly increased expression of genes in cell regulation and development of apoptosis, respectively. In summary, CB vs. PB CD56 dim NK cells appear to be earlier in development, have decreased functional activity, and increased capacity for programmed cell death, suggesting that CB NK cells require functional and maturational stimulation to achieve similar functional levels as PB CD56 dim NK cells.

【 授权许可】

CC BY|CC BY-NC