LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3

Innate immunity

article

Peipei Fang¹  Yiping Chen²  Chenwei Pan¹  Luxia Xiang¹  Weilai Chen³  Shaoxun Li¹  Shanshan Huang¹  Jie Li¹  Lu Zhuge¹  Lingxiang Jin¹  Wenke Feng² 

[1] Department of Infectious Disease, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University;Pediatric Hepatitis and Liver disease Clinical Center, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University;Department of Neurology, Wenzhou People’s Hospital;The Second School of Medicine, Wenzhou Medical University

关键词: lncRNA GAS5;    miR-544;    RUNX3;    natural killer cell;    liver cancer;   

DOI  :  10.1177/1753425919827632

来源: Sage Journals

【 摘 要 】

This study aimed to explore the role of lncRNA GAS5 in the regulation of the killing effect of NK cells on liver cancer. Compared with a control group, lncRNA GAS5, RUNX3, and NCR1 were down-regulated in NK cells of patients with liver cancer, whereas miR-544 expression was up-regulated in NK cells of patients with liver cancer. Activated NK cells had higher IFN-γ level. Knockdown of GAS5 in activated NK cells decreased IFN-γ secretion, NK cell cytotoxicity, the percentage of CD107a+ NK cells, and the apoptosis rate of HepG2 and Huh7 cells. We also proved the interaction of GAS5 and miR-544, and the negative regulation role of GAS5 on miR-544. GAS5 overexpression in activated NK cells increased RUNX3 expression, IFN-γ secretion, the NK cell cytotoxicity, the percentage of CD107a+ NK cells, and the apoptosis rate of HepG2 cells, while miR-544 mimic abolished the promotion effect of GAS5 overexpression. Finally, in vivo experiments indicated an inhibition effect of GAS5 in tumor growth. LncRNA GAS5 overexpression enhances the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3.

【 授权许可】

CC BY|CC BY-NC