Differential expression profiles of miRNAs and their putative targets in Schistosoma mansoni during its life cycle

Memorias do Instituto Oswaldo Cruz

article

Fabiano CP Abreu¹  Ester Alves Mota¹  Roberta V Pereira¹  Victor F Oliveira¹  Marcela P Costa¹  Matheus de S Gomes²  Liana K Jannotti-Passos³  William C Borges¹  Renata Guerra-Sá¹ 

[1] Universidade Federal de Ouro Preto;Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica;Fundação Oswaldo Cruz-Fiocruz, Centro de Pesquisas René Rachou

关键词: miRNA;    differential expression;    Schistosoma mansoni;    miRNA target genes;   

DOI  :  10.1590/0074-02760200326

学科分类：地质学

来源: Bioline International

【 摘 要 】

BACKGROUND Schistosomiasis is a disease caused by Schistosoma. Due to its complex life cycle, evolutionary position and sexual dimorphism, schistosomes have several mechanisms of gene regulation. MicroRNAs (miRNAs) are short endogenous RNAs that regulate gene expression at the post-transcriptional level by targeting mRNA transcripts. OBJECTIVES Here, we tested 12 miRNAs and identified their putative targets using a computational approach. METHODS We performed the expression profiles of a set of miRNAs and their putative targets during the parasite’s life cycle by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). FINDINGS Our results showed differential expression patterns of the mature miRNAs sma-miR-250; sma-miR-92a; sma-miRnew_4-3p; sma-miR-new_4-5p; sma-miR-new_5-5p; sma-miR-new_12-5p; sma-miR-new_13-3p and sma-miR-new_13-5p. Interestingly, many of the putative target genes are linked to oxidative phosphorylation and are up-regulated in adult-worms, which led us to suggest that miRNAs might play important roles in the post-transcriptional regulation of genes related to energetic metabolism inversion during parasite development. It is noteworthy that the expression of sma-miR-new_13-3p exhibited a negative correlation on SmNADH:ubiquinone oxidoreductase complex I. MAIN CONCLUSIONS Our analysis revealed putative miRNA genes related to important biological processes, such as transforming growth factor beta (TGF-β) signaling, proteasome regulation, glucose and lipid metabolism, immune system evasion and transcriptional regulation.

【 授权许可】

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