Integrated DNA Methylation and Gene Expression Analysis Identified S100A8 and S100A9 in the Pathogenesis of Obesity

Frontiers in Cardiovascular Medicine

article

Ningyuan Chen¹  Liu Miao²  Wei Lin³  Donghua Zou⁴  Ling Huang¹  Jia Huang⁵  Wanxin Shi⁵  Lilin Li⁵  Yuxing Luo⁵  Hao Liang⁵  Shangling Pan¹  Junhua Peng¹ 

[1] Department of Pathophysiology, School of Preclinical Medicine, Guangxi Medical University;Department of Cardiology, Liuzhou People's Hospital, Guangxi Medical University;Department of Neurological Rehabilitation, Guangxi Jiangbin Hospital;Department of Neurology, The Fifth Affiliated Hospital of Guangxi Medical University;The First Clinical Medical School, Guangxi Medical University

关键词: obesity;    DNA methylation;    mRNA expression;    function enrichment;    correlation analyses;   

DOI  :  10.3389/fcvm.2021.631650

学科分类：地球科学(综合)

来源: Frontiers

【 摘 要 】

Background: To explore the association of DNA methylation and gene expression in the pathology of obesity. Methods: (1) Genomic DNA methylation and mRNA expression profile of visceral adipose tissue (VAT) were performed in a comprehensive database of gene expression in obese and normal subjects. (2) Functional enrichment analysis and construction of differential methylation gene regulatory networks were performed. (3) Validation of the two different methylation sites and corresponding gene expression was done in a separate microarray dataset. (4) Correlation analysis was performed on DNA methylation and mRNA expression data. Results: A total of 77 differentially expressed mRNAs matched with differentially methylated genes. Analysis revealed two different methylation sites corresponding to two unique genes—s100a8-cg09174555 and s100a9-cg03165378. Through the verification test of two interesting different expression positions [differentially methylated positions (DMPs)] and their corresponding gene expression, we found that methylation in these genes was negatively correlated to gene expression in the obesity group. Higher S100A8 and S100A9 expressions in obese subjects were validated in a separate microarray dataset. Conclusion: This study confirmed the relationship between DNA methylation and gene expression and emphasized the important role of S100A8 and S100A9 in the pathogenesis of obesity.

【 授权许可】

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