Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales

Frontiers in Medicine

article

Rémy A. Bonnin¹  Agnès B. Jousset¹  Cécile Emeraud¹  Saoussen Oueslati¹  Laurent Dortet¹  Thierry Naas¹ 

[1] Université Paris-Saclay, LabEx LERMIT, Faculty of Medicine;Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae;Evolution and Ecology of Resistance to Antibiotics Unit, Institut Pasteur-APHP-Université Paris-Sud;Bacteriology-Hygiene Unit, Bicêtre Hospital

关键词: CHDL;    carbapenem;    transposon;    insertion sequence;    antibiotic resistance;    detection;   

DOI  :  10.3389/fmed.2020.616490

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Gram-negative bacteria, especially Enterobacterales, have emerged as major players in antimicrobial resistance worldwide. Resistance may affect all major classes of anti-gram-negative agents, becoming multidrug resistant or even pan-drug resistant. Currently, β-lactamase-mediated resistance does not spare even the most powerful β-lactams (carbapenems), whose activity is challenged by carbapenemases. The dissemination of carbapenemases-encoding genes among Enterobacterales is a matter of concern, given the importance of carbapenems to treat nosocomial infections. Based on their amino acid sequences, carbapenemases are grouped into three major classes. Classes A and D use an active-site serine to catalyze hydrolysis, while class B (MBLs) require one or two zinc ions for their activity. The most important and clinically relevant carbapenemases are KPC, IMP/VIM/NDM, and OXA-48. However, several carbapenemases belonging to the different classes are less frequently detected. They correspond to class A (SME-, Nmc-A/IMI-, SFC-, GES-, BIC-like…), to class B (GIM, TMB, LMB…), class C (CMY-10 and ACT-28), and to class D (OXA-372). This review will address the genetic diversity, biochemical properties, and detection methods of minor acquired carbapenemases in Enterobacterales.

【 授权许可】

CC BY