In vitro Infectivity of Strains Isolated From Dogs Naturally Infected With Leishmania infantum Present a Distinct Pathogenic Profile in Hamsters

Frontiers in Medicine

article

Lucilene Aparecida Resende¹  Jaqueline Costa Leite¹  Patricia Silveira¹  Tatiane Furtado de Carvalho³  Renato Lima Santos³  Denise da Silveira-Lemos¹  Olindo Assis Martins-Filho⁴  Walderez Ornelas Dutra¹  Alexandre Barbosa Reis²  Rodolfo Cordeiro Giunchetti¹  Rodrigo Dian de Oliveira Aguiar-Soares²  Nádia das Dores Moreira²  Sidney de Almeida Ferreira²  Mariana Ferreira Lanna¹  Jamille Mirelle de Oliveira Cardoso²  Fernando Augusto Siqueira Mathias²  Wendel Coura-Vital²  Reysla Maria da Silveira Mariano¹ 

[1] Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais;Laboratório de Imunopatologia, Universidade Federal de Ouro Preto;Departamento de Clínica e Cirurgia Veterinárias, Universidade Federal de Minas Gerais;Instituto René Rachou;Departamento de Medicina, Universidade José Do Rosário Vellano;Departamento de Análises Clínicas, Escola de Farmácia, Universidade Federal de Ouro Preto;Programa de Pós Graduação em Ciências Farmacêuticas (Cipharma), Escola de Farmácia, Universidade Federal de Ouro Preto;Laboratório de Biologia Parasitária, Universidade Federal de Lavras;Laboratório de Pesquisa em Epidemiologia e Citologia, Departamento de Análises Clínicas, Escola de Farmácia, Universidade Federal de Ouro Preto

关键词: Leishmania infantum;    visceral leishmaniasis;    experimental infection;    pathogenicity;    infectivity;   

DOI  :  10.3389/fmed.2020.00496

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Visceral leishmaniasis (VL) is a severe disease caused by Leishmania infantum . Dogs are the parasite's main reservoir, favoring its transmission in the urban environment. The analysis of L. infantum from infected dogs contributes to the identification of more virulent parasites, thereby supporting basic and applied studies such as vaccinal and therapeutic strategies. We proposed the in vitro and in vivo characterization of L. infantum strains from naturally infected dogs from a VL endemic area based on an infectivity and pathogenicity analysis. DH82 canine macrophages were infected in vitro with different strains for infectivity analysis, showing distinct infectivity profiles. The strains that showed greater and lesser infectivity using in vitro analyses (616 and 614, respectively) were used to infect hamsters for pathogenicity analysis. The group infected with strain 616 showed 100% survival while the group infected with strain 614 showed 50% after seven months of follow up. Furthermore, the 614 strain induced more noticeable clinicopathological changes and biochemical abnormalities in liver function, along with high inflammation and parasite load in the liver and spleen. We confirmed high variability of infectivity and pathogenicity in L. infantum strains from infected dogs. The results support the belief that screening for L. infantum infectivity using in vitro experiments is inadequate when it comes to selecting the most pathogenic strain.

【 授权许可】

CC BY