Effects of Dietary Fat Profile on Gut Microbiota in Valproate Animal Model of Autism

Frontiers in Medicine

article

Jin-peng Wang¹  Ping Li²  Yang-chun Xu³  Ji-qiu Hou⁴  Jia-yu Li⁵  Jie Xing²  Bao-xia Yang²  Ze-hui Zhang⁵  Bei-lin Zhang⁵  Hong-hua Li⁶ 

[1] Department of Cardiology, The Second Hospital of Jilin University;Department of Developmental Pediatrics, The Second Hospital of Jilin University;Department of Dermatology, The Second Hospital of Jilin University;Department of Pharmacy, The Second Hospital of Jilin University;Department of Physiology, College of Basic Medical Sciences, Jilin University;Department of Developmental and Behavioral Pediatrics, The First Hospital of Jilin University

关键词: autism spectrum disorder;    gastrointestinal;    fatty acid;    microbiota;    behavior;    valproate acid;   

DOI  :  10.3389/fmed.2020.00151

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Autism spectrum disorder (ASD) is a developmental disability which may cause significant social, communication, and behavioral challenges. Besides certain essential symptoms, a lot of ASD individuals also suffer the comorbidity of gut microbiota dysbiosis, which possibly causes a variety of gastrointestinal (GI) difficulties. Interestingly, evidence has indicated that behavioral output may be modulated through the communication between the central nervous system and gut microbiota via the gut-brain axis. Polyunsaturated fatty acids (PUFAs) and n-3 fatty acids (n-3 PUFA) are structurally and functionally crucial components for the brain, and the state of n-3 PUFAs also affects the gut microbiota. However, how varying intake ratios of n-3/n6 PUFAs affect the gut microbiota composition in ASDs is not well-understood. Pregnant female Wistar rats with intraperitoneal administration of valproate acid (VPA) at embryonic day (E) 12.5 and their male offspring were grouped and fed three diets: a control chow (VPA group), omega-3 deficient (A group), and n-3/n6 (1:5) diet (B group). The diet of pregnant female Wistar rats with intraperitoneal administration of saline and their male offspring was a control chow (normal group). Microbial composition and species abundance were investigated accordingly by the 16S rRNA gene-based metagenomics analysis on the fecal samples. Results showed that fecal microbial abundance was decreased because of VPA administration in the period of pregnancy, and the changing pattern of gut microbiota was similar to that reported in ASD patients. Furthermore, the n-3/n6 (1:5) diet increased the fecal microbial abundance and decreased the elevated Firmicutes. In conclusion, n-3/n6 PUFAs (1:5) diet supplementation may alter gut microbiota composition in VPA-exposed rats. This study put forward a new strategy for the intervention and treatment of autism by n-3/n-6 PUFAs ratio supplementation intakes.

【 授权许可】

CC BY