New Developments in Transcriptomic Analysis of Synovial Tissue

Frontiers in Medicine

article

Hayley L. Carr¹  Jason D. Turner¹  Triin Major¹  Dagmar Scheel-Toellner¹  Andrew Filer² 

[1] Institute for Inflammation and Ageing, University of Birmingham, United Kingdom;NIHR Birmingham Biomedical Research Centre, University Hospitals Birmingham NHS Foundation Trust, United Kingdom

关键词: transcriptomics;    synovium;    sequencing;    single-cell;    microarray;    stratification;    fibroblast;    pathotype;   

DOI  :  10.3389/fmed.2020.00021

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Transcriptomic technologies are constantly changing and improving, resulting in an ever increasing understanding of gene expression in health and disease. These technologies have been used to investigate the pathological changes occurring in the joints of rheumatoid arthritis patients, leading to discoveries of disease mechanisms, and novel potential therapeutic targets. Microarrays were initially used on both whole tissue and cell subsets to investigate research questions, with bulk RNA sequencing allowing for further elaboration of these findings. A key example is the classification of pathotypes in rheumatoid arthritis using RNA sequencing that had previously been discovered using microarray and histology. Single-cell sequencing has now delivered a step change in understanding of the diversity and function of subpopulations of cells, in particular synovial fibroblasts. Future technologies, such as high resolution spatial transcriptomics, will enable step changes integrating single cell transcriptomic and geographic data to provide an integrated understanding of synovial pathology.

【 授权许可】

CC BY