Clinical and Microbiological Characteristics of a Community-Acquired Carbapenem-Resistant Escherichia coli ST410 Isolate Harbouring bla NDM-5-Encoding IncX3-Type Plasmid From Blood

Frontiers in Medicine

article

Ji-Na Gu¹  Lin Chen¹  Xing-Bei Weng²  Xiao-Yan Yang¹  Dan-Mei Pan¹ 

[1] Department of Infectious Medicine, Hwa Mei Hospital, University of Chinese Academy of Sciences;Department of Laboratory Medicine, Ningbo First Hospital

关键词: whole genome sequence;    Escherichia coli ST410;    NDM-5;    IncX3-type plasmid;    high-risk clone;   

DOI  :  10.3389/fmed.2021.658058

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Objectives: The aim of this research was to investigate the clinical and microbiological characteristics of a case of community-acquired carbapenem-resistant Escherichia coli isolated from a patient with a bloodstream infection in China. Methods:Escherichia coli Huamei202001 was recovered from the first blood culture from a patient hospitalised in China. An antimicrobial susceptibility test was performed, and the genome was sequenced on an Illumina HiSeq X 10 platform with a 150-bp paired-end approach. The generated sequence reads were assembled using Unicycler, and the whole genome sequence data were analysed using bioinformatics tools. Moreover, the patient and her main family members obtained a faecal sample screening test for CRE, the positive strain was further isolated and the identification and antimicrobial susceptibility testing was performed. Results:Escherichia coli Huamei202001 belonged to sequence type 410. In addition, a bla NDM-5-encoding IncX3-type plasmid was responsible for the spreading of carbapenem resistance. Only the patient was detected as having a positive faecal sample screening test for CRE. Strain Fec01 was identified as E. coli , and the antibiotic susceptibility profile was the same as that of E. coli Huamei202001. Conclusions:Escherichia coli Huamei202001 is defined as community-acquired carbapenem-resistant Enterobacteriaceae. The clone ST410 that harbours the bla NDM-5-encoding IncX3-type plasmid is causing new high-risk clones globally. Thus, infection control measures should be strengthened to curb the dissemination of IncX3.

【 授权许可】

CC BY