Inflammation Biomarkers and Correlation to Wound Status After Full-Thickness Skin Grafting

Frontiers in Medicine

article

Karim Saleh¹  Ann-Charlotte Strömdahl¹  Kristian Riesbeck²  Artur Schmidtchen¹ 

[1] Division of Dermatology, Department of Clinical Sciences, Skane University Hospital, Lund University;Clinical Microbiology, Department of Translational Medicine, Faculty of Medicine, Lund University;Department of Biomedical Sciences, Copenhagen Wound Healing Center, Bispebjerg Hospital, University of Copenhagen

关键词: dermatologic surgery;    wound healing;    surgical site infection;    full-thickness skin grafting;    inflammation biomarkers;   

DOI  :  10.3389/fmed.2019.00159

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

Background: A surgical site infection (SSI) is believed to be the result of an exaggerated inflammatory response. Objective: Examine the relationship between clinical status and inflammation biomarkers in full-thickness skin grafting wounds. Methods: Twenty patients planned for facial full-thickness skin grafting were enrolled. A week after surgery, all graft wounds were clinically assessed using a 3-step scale for inflammation (low, moderate, high). All wounds were swabbed for routine microbiological analysis and assessment of numbers of aerobic bacteria. Tie-over dressings from all patients were collected and used for wound fluid extraction and subsequent analysis of MMPs, cytokines, and NF-κB inducing activity. Results: Wounds with a high degree of inflammation contained increased total MMP activity ( P ≤ 0.05) in their corresponding fluids. Likewise, the level of the cytokines IL-1ß, IL-8, IL-6, TNF-α was analyzed, and particularly IL-1ß was discriminatory for highly inflamed wounds ( P ≤ 0.01). Moreover, bacterial loads were increased in highly inflamed wounds compared to wounds with a low degree of inflammation ( P ≤ 0.01). NF-κB activation in the monocytic cell line THP-1 was significantly higher when these cells were stimulated by wound fluids with a high degree of inflammation ( P ≤ 0.01). Growth of S. aureus in wounds did not vary between wounds with different degrees of inflammation (chi-square 3.8, P = 0.144). Conclusion: Biomarkers analyzed from tie-over dressings correlated to clinical wound healing in full-thickness skin grafting.

【 授权许可】

CC BY