Nine Cases of Methanogenic Archaea in Refractory Sinusitis, an Emerging Clinical Entity

Frontiers in Public Health

article

Elisabeth Sogodogo¹  Mustapha Fellag¹  Ahmed Loukil¹  Vanessa Demonfort Nkamga¹  Justin Michel²  Patrick Dessi²  Pierre-Edouard Fournier¹  Michel Drancourt¹ 

[1] Aix Marseille University;Hôpital de la Conception

关键词: Methanobrevibacter oralis;    Methanobrevibacter smithii;    Methanobrevibacter massiliense;    sinusitis;    genotyping;    archaea;    methanogen;   

DOI  :  10.3389/fpubh.2019.00038

学科分类：社会科学、人文和艺术（综合）

来源: Frontiers

【 摘 要 】

The authors report the cases of 9 patients eventually diagnosed with methanogenic archaea refractory or recalcitrant chronic rhinosinusitis, a condition known to involve various anaerobic bacteria but in which the role of methanogenic archaea is unknown. The authors retrospectively searched these microorganisms by PCR in surgically-collected sinusal pus specimens from patients diagnosed with refractory sinusitis, defined by the persistance of sinus inflammation and related-symptoms for more than 12 weeks despite appropriate treatment. Of the 116 tested sinus surgical specimens, 12 (10.3%) from 9 patients (six females, three males; aged 20–71 years) were PCR-positive. These specimens were further investigated by fluorescence in-situ hybridization, PCR amplicon-sequencing and culture. Methanobrevibacter smithii was documented in four patients and Methanobrevibacter oralis in another four, one of whom was also culture-positive. They were associated with a mixed flora including Gram-positive and Gram-negative bacteria. In the latter patient, “ Methanobrevibacter massiliense ” was the sole microorganism detected. These results highlight methanogenic archaea as being part of a mixed anaerobic flora involved in refractory sinusitis, and suggest that the treatment of this condition should include an antibiotic active against methanogens, notably a nitroimidazole derivative.

【 授权许可】

CC BY